中文摘要：

目的诱导骨髓间充质干细胞（bone marrow mesenchymal stem cells,BM-MSCs）分化构建类肾器官。方法通过CHIR99021与FGF9两种细胞因子的调控,利用BM-MSCs分化构建类肾器官。对类肾器官行免疫荧光染色,电镜观察类肾器官的结构。结果通过调控细胞因子,成功利用BM-MSCs分化获得类肾器官。在类肾器官中,单个肾单位分为远端小管、近端小管以及肾小球,肾小球中有足细胞和血管。结论利用BM-MSCs构建的类肾器官是极佳的肾器官模型,未来可应用于肾毒性药物筛选、疾病模型、肾支架灌注。

英文摘要：

Objective To induce bone marrow-derived mesenchymal stem cells（BM-MSCs） to differentiate into kidney organoids. Methods By regulating CHIR99021 and FGF9, BM-MSCs were used to differentiate into the kidney organoids. Kidney organoids were treated with immunofluorescence staining. Then electron microscope was used to observe the structure of the kidney organoids. Results By regulating CHIR99021 and FGF9, we have generated the kidney organoids from BM-MSCs successfully. Within these kidney organoids, single renal unit segmented into distal tubules, proximal tubules and glomeruli, which contained podocytes and blood vessels. Conclusion Such kidney organoids generated from BM-MSCs represent powerful models of the human organ which can be used for future clinical research, including nephrotoxicity screening, disease modelling and kidney scaffolds recellularising.