中文摘要：

下丘脑作为神经活动中枢具有调控能量平衡和生殖的重要作用。CYP17A1、类固醇激素急性调节蛋白（St AR）和1型蛋白（HSD3B1）作为对生殖过程中类固醇激素合成过程的关键调控因子,其在小鼠下丘脑和卵巢中表达部位尚不明确。本研究应用免疫荧光检测了小鼠下丘脑中上述调节因子的神经元表达位置和在卵巢中的分布情况。结果显示,CYP17A1在小鼠的下丘脑腹内侧核和穹窿处具有区域性分布的同时也表达在成熟的黄体组织中。HSD3B1主要表达在卵泡的颗粒细胞,St AR与CYP17A1在卵母细胞中存在共表达关系。上述结果提示,下丘脑中CYP17A1在下丘脑-垂体-性腺轴中,并可能参于调控小鼠卵巢类固醇激素的合成;St AR,HSD3B1不仅存在于下丘脑的神经元中,也同样存在于卵巢组织中,参与类固醇激素的合成。本文为进一步研究下丘脑调控卵巢合成类固醇激素的生理过程提供了基础。

英文摘要：

Hypothalamus plays a key role in mice energy balance and reproduction.CYP17A1,St AR and HSD3B1 are key factors in mouse steroidogenic,but little evidence describes the expression of CYP17A1,St AR and HSD3B1 in mouse hypothalamus and ovary.The results showed that CYP17A1 was expressed in ventromedial hypothalamic nucleus and fornix area,while it was also expressed in lutein cells in mouse ovary.HSD3B1 was mainly expressed in granulosa cells.In the oocyte,CYP17A1 co-localized with St AR.These results suggest that CYP17A1 may regulate ovary metabolism and synthesis of steroid hormones in mice.We also demonstrated that in CYP17A1 existed hypothalamus-pituitary-gonadal axis in rodents and oocyte takes part in the steroid hormones synthesis.St AR and HSD3B1 were also localized in female mouse ovary and might play a key role in mouse steroid hormones synthesis.This study provides a basis for the study of hypothalamus to regulate ovary to synthase steroid hormones in the future.