中文摘要：

目的观察RNA干扰Ras相关C3肉毒素底物1（Racl—siRNA）重组载体对视网膜新生血管生成的抑制作用。方法25只成年Sprague-Dawley（SD）大鼠，采用光动力疗法诱导双眼视网膜静脉阻塞后，1只眼玻璃体腔转染Racl-siRNA重组载体作为基因干预组，另1只眼注射空白载体作为空白对照组。25只SD大鼠单眼玻璃体腔内转染Racl—siRNA重组载体作为空白干预组。2周后对3组大鼠进行心内灌注右旋异硫氰酸荧光素，视网膜铺片，检测视网膜新生血管生成情况。摘除3组大鼠眼球，苏木素-伊红染色后计数突破内界膜的内皮细胞数。结果视网膜铺片发现，空白对照组视网膜产生大片新生血管，大量荧光素渗漏；基因干预组仅见小片状新生血管网，少量荧光素渗漏；空白干预组呈正常血管形态。基因干预组大鼠视网膜切片中，突破内界膜的血管内皮细胞平均数与空白对照组和空白干预组比较，两两间差异具有统计学意义（F=47．168，P=0．000）。结论Racl-siRNA重组载体能有效抑制体内视网膜新生血管的生成。

英文摘要：

Objective To evaluate the inhibited effects of small interfering RNA targeting Racl （Rael-siRNA） on rat retinal neovascularization in retinae. Methods Retinal vein occlusion was induced by retinal photodynamic medthod in 25 Sprague Dawley rats. Racl-siRNA vector DNA was injected into the vitrous of one eye of those rats （gene intervention group）, and empty vector DNA was injected into the fellow eye （blank control group）. Racl siRNA vector was injected in other 95 SD rats without retinal vein occlusion （blank intervention group）. Two weeks after injection, fluorescein isothiocyanate （FITC） dextran was perfused into the hearts of all the rats, and the retinal wholemount was made to observe the neovascularization. The numbers of endothelial cells which break through the internal limiting membrane were counted after hematoxylin eosin staining. Results A massive of neovascularization and FITC; leakage were found in blank control group. Small part of neovascularization and a little FITC leakage were observed in the gene intervention group. Retinal vessels were normal in blank intervention group. Compared with blank contrast group and blank intervention group, the difference of the mean numbers of endothelial cells which broke through the internal limiting membrane in the gene intervention group was significant （F =- 47. 168, P = 0. 000）. Conclusion Racl siRNA can inhibit retinal neovascularization induced by retinal vein occlusion in rats.