以田艾绒为原料,采用微波辅助乙醇法提取田艾中总黄酮。在单因素试验基础上,选择乙醇体积分数、微波功率、微波时间为自变量,总黄酮得率为响应值,采用响应面法中的Box-Behnken模型对田艾中总黄酮提取工艺进行优化。结果表明,最佳提取工艺条件如下:料液比为1∶50(g/m L),预浸时间为30 min,乙醇体积分数为20%,微波功率为350 W,微波时间为67 s,此条件下田艾总黄酮的实际得率为23.60 mg/g。
With Tian moxa as raw material,using microwave auxiliary ethanol method to extract the total flavonoids in Tian Ai.On the basis of single factor experiment,the ethanol concentration,microwave power and time was chose as the independent variable and the total flavonoids yield was used as response value,and the extraction process of total flavonoids in Tian Ai was optimized by response surface methodology of Box-Behnken mode.The results showed that the optimum extraction technology conditions were solid-liquid ratio for 1 ∶ 50(g/m L),presoak time for 30 min,ethanol concentration for 20 %,microwave power was 350 W,microwave time of 67 s.Under the condition,the actual extraction yield of Tian Ai total flavonoids was 23.60 mg/g.