中文摘要：

目的:动态观察氟哌啶醇季铵盐衍生物（F₃）对血管平滑肌细胞内钙浓度的影响。方法:利用激光共聚焦显微镜动态观察F₃（0.o1-10μmol/L）对由KCl（30mmol/L）诱导的大鼠血管平滑肌细胞钙荧光强度增加的作用。结果:KCl可诱发细胞内钙荧光强度迅速增强,F₃可以拮抗由KCl诱导的细胞内钙荧光强度增强作用,并呈量效依赖性和时间依赖性,终强度（KCl:67±24;F₃ 0.o1μmol/L:57±13;0.1μmol/L:40±13;1μmol/L:29±9;10μmol/L:20±6）。在加入F₃后,钙荧光强度的变化最快时程是在给F₃后0-30s。结论:F₃拮抗血管收缩主要是由于阻断了Ca²⁺通道。

英文摘要：

AIM: To study the effect of quaternary ammonium salt derivative of haloperidol (F3) on intracellular calcium (Ca2+ ) dynamic change in vascular smooth muscle cells (VSMC). METHODS: Using laser confocal scanning microscopy (LCSM), to observe the effect of F3(0.01 -10μmol/L) on increase of intracellular Ca2 + fluorescent intensity induced by KC1 (30 mmol/L) in VSMC. RESULTS: KC1 induced a rapid increase of intracellular Ca2 + fluorescent intensity. F3 inhibited the rapid increase of that in both concentration-dependent manner (KC1: 67 +24; F3 0.01μmol/L: 57 +13; 0.1μmol/ L: 40+13; 1μmol/L: 29+9; 10μmol/L: 20+6) and time-dependent manner; during 0 - 30 s after F3 was added, the change of fluorescent intensity was more rapid. CONCLUSION: The inhibitory effect of F3 on Ca2+ concentration in VSMC was mainly due to the blocking of the Ca2+ influx, and might partially attributed to opening of the potassium (K+ ) channel.