中文摘要：

为确定江西某猪场部分母猪陆续发生皮肤痘斑的病原，根据猪痘病毒（AF410153．1）基因序列设计1对引物，采用PCR方法从发病母猪皮肤痘痂中扩增到猪痘病毒P35基因，与猪痘病毒参考毒株（AF410153．1）P35基因的核苷酸同源性为98％，说明该场母猪皮肤出现的痘斑是由猪痘病毒引起。利用PK15细胞从皮肤痘痂中分离到1株猪痘病毒，该毒株能在PK15细胞中连续传代，不产生严重的细胞病变（CPE）。采用间接ELISA方法对该场的10头母猪和8头肥猪血样进行猪痘病毒抗体检测，母猪和肥猪血清中猪痘病毒抗体阳性数分别为5头和4头，阳性率为50％。

英文摘要：

In order to identify the etiologic agents of eruptive dermatitis （ papulae, crusts, et al） in sows in Jiangxi Province, a pair of primers were designed according to the swinepox virus （ SWPV ） genome （ AF410153.1 ）. Crusts colleted from skin of sows were used for DNA extraction, followed by PCR detection of SWPV P35 gene. The amplified Nucleotide Sequence shared 98% identity with the reported SWPV P35 gene （ AF410153.1 ）. The results indicated that the etiologic agent of eruptive dermatitis is Swinepox virus. A strain of Swiriepox virus was isolated by PK15 cell culture. The SWPV could be replicated in PK15 ceils without severe cytopathogenic effects （CPE）. The antibodies against SWPV P35 protein in 18 sera of sows and finishing pigs collected from the farm were detected by the indirect ELISA, and the results showed that 50% samples were positive, indcluding 8 sows and 4 finishing pigs.