中文摘要：

目的 研究培养于载玻片上的破骨细胞内Ca^2＋的空间分布情况。方法 体外培养获得破骨细胞，采用抗酒石酸酸性磷酸酶染色和扫描电镜观察骨吸收情况鉴定破骨细胞；应用激光扫描共聚焦显微镜和Fluo-3／AM荧光探针标记技术，测定了破骨细胞胞内游离Ca^2＋的分布，获得的图像在计算机内用图像分析软件分析。结果 在37℃、Fluo-3／AM终浓度为10μmol／L的条件下，破骨细胞可获得良好的标记效果，破骨细胞中部层面内ca2＋荧光信号的强度较高，同一层面内分布是不均匀的。结论 破骨细胞的不同位置的细胞器内或其附近的Ca^2＋浓度不同，破骨细胞可通过Ca2慷度的空间差异来调节其自身功能。

英文摘要：

Objective To study the spatial distribution of free Ca^2＋ in osteoclast-like cells cultured on glass. Methods To detect the free Ca^2＋ in osteoclast-like cells, the images were analyzed with image software, using the laser scanning confocal microscope and fluorescent probe. Results At 37℃ the free Ca^2＋ in osteoclast-like cells could be labelled effectively with 10 μmol/L Fluo-3/AM, the intensity of Ca^2＋ fluorescent signal in the central part was greater than that in the peripheral part and in the same section the signal was not distributed evenly. Conclusion The intensity of Ca^2＋ fluorescent signal is different among various organellae in osteoclast-like cell, which suggests the osteoclast-like cell modulate its own function through the spatial difference of free Ca^2＋ concentration.