目的:建立复方红景天口含片中二苯乙烯苷的含量测定方法。方法:色谱柱:Agilent TC-C18柱(4.6 mm×250mm,5μm),流动相:乙腈∶水(15∶85),流速:1.0 mL/min,检测波长:320 nm。结果:二苯乙烯苷在0.162~1.944μg范围内与吸收峰峰面积积分值呈良好的线性关系,r=0.999 3;平均加样回收率为100.34%,RSD为1.75%(n=9)。结论:该方法简单、快速、准确、重现性好,可用于复方红景天口含片的质量控制。
Objective: To establish a method for content determination of stilbene glycoside in compound herba rhodiolae buccal tablets.Method: Chromatographic column: Agilent TC-C18 column(4.6 mm×250 mm,5 μm),mobile phase: acetonitrile: water(15: 85),the flow rate: 1.0 mL/min,the detection wavelength was 320nm.Result: Stilbene glycoside shows a good linear relationship in the range of 0.162-1.944 μg and integral value of absorption peak area,r=0.999 3;the mean recovery was 100.34%,RSD=1.75%(n=9).Conclusion: The method,simple,rapid,accurate with good repeatability,can be used for quality control of compound herba rhodiolae buccal tablets.