中文摘要：

目的观察血晶素（heroin）对缺氧缺糖（0GD）海马脑片损伤的保护作用及其对血红素加氧酶1（heme oxygenase-1，HO-1）蛋白表达的影响。方法取新生8～10dSD大鼠海马脑片，培养2周。将脑片分为正常培养对照组（HOTC），缺氧缺糖组（0GD），预加Hemin再缺氧缺糖组（Heroin＋OGD），预加HO-1抑制剂锌原卟啉（Znpp）再缺氧缺糖组（Znpp＋OGD）。应用免疫组织化学染色和图像分析处理技术观察海马脑片HO-1蛋白表达，并观察神经元和星形胶质细胞形态学改变。结果HO-1在正常海马脑片的神经元和星形胶质细胞中均有弱阳性表达。与对照组比较，OGD组海马已失去正常结构，锥体细胞发生坏死，HO-1蛋白表达增强。与OGD组相比，Heroin＋0GD组海马脑片形态基本正常，锥体细胞数较多，H01蛋白的表达明显增强。Znpp＋OGD组海马结构消失，HO-1蛋白表达明显减弱。结论血晶素对缺氧缺糖海马脑片损伤有明显的保护作用，其机制可能与其诱导神经元和星形胶质细胞表达HO-1蛋白密切相关。

英文摘要：

Objective To investigate the neuroprotedive role of heroin and its effect on the expression of heme oxygenase 1 （HO 1） in the hippoeampal organotypic tissue cultures （HOTC） after combined oxygen/glucose deprivation（OGD）. Methods SD rats,8--10d old, were sacrificed for brain slices. HOTC were made and randomly divided into four groups： HOTC, HOTC undergoing OGD （OGD）, HOTC undergoing OGD following pretreatment with heroin （Heroin＋ OGD） ; HOTC undergoing OGD following pretreatment with Znpp（Znpp＋ OGD）. The expression of HO-1 was detected by immunohistochemical technique and computer-assisted image analysis. Results HO-1 expression was observed in the neurons and gliaeytes in HOTC group. In OGD group,hipp ocampal organotypic tissue lost normal structure and neurons were damaged severely. Pretreatment with heroin could effectively reverse the damage of the neurons,and pretreatment with Znpp,a HO-1 inhibitor,could aggravate the damage. The expression of HO-1 protein in the Hemin＋OGD group was significantly stronger than that in the OGD group. The expression of HO-1 in Znpp＋ OGD group was significantly weaker than that in the OGD group. Conclusion Heroin has the protective effect on injury induced by OGD in rat hippaoeampal slices,which is associated with enhanced expression of HO-1 in neurons and gliaeytes.