中文摘要：

以中华补血草叶片为材料,提取其总RNA并反转录cDNA,并以cDNA为模板,克隆得到包含该基因完整开放阅读框的cDNA序列,序列分析表明,该基因开放阅读框长249 bp,编码82个氨基酸;该蛋白含有14个半胱氨酸,主要分布在蛋白质的N端和C端,呈CC,CX,CXXC形式排列.预测该蛋白分子质量为8 133.1 D;等电点为4.72;35～48位和52～68位氨基酸间有2个较强的疏水区.用3%NaCl处理补血草植株0、12、244、8、72h,利用半定量-PCR技术对该基因在盐胁迫下表达模式的分析表明,在3%NaCl处理条件下,随着盐处理处理时间的延长该基因的表达量逐渐升高,在盐处理48 h时表达量最高,之后其表达量出现下降.

英文摘要：

The metallothionein gene,with an open reading frame of 249bp and encodes a protein of 82 amino acid residues,is cloned from Limonium sinense Kuntze.This protein has 14 Cys residues（Cys） and a conserved sequence of C-C,C-X-C,C-X-X-C at the end of both C-and N-terminal.There are two strong hydrophobic areas at 35-48 and 52-68 amino acid residues.Expression analysis of MT by semi-quantitative-PCR technology showes the expression level is increasing gradually when Limonium sinense Kuntze is treated with 3% NaCl for 0,12,24,48,72 h.It reaches the highest levels at 48 h,then decreases.This study lays a foundation for the deep research of MT from Limonium sinense Kuntze in response to salt stress.