中文摘要：

利用花粉管通道法将辣椒总DNA导入小麦,获得T0代种子。T_1代播种后表型总变异率为21.3%,对照组为4%;变异谱有9种,对照组只有2种。无论在变异谱上还是变异率上,经辣椒总DNA转化的小麦材料与对照组有着明显的差异。随机选取转化材料中的47株材料主茎旗叶（尖部1/4）,提取DNA进行IRAP分析,共扩增条带6条,有5条存在多态性。花粉管通道法向小麦中导入远缘辣椒总DNA能够在当代引起多种变异,并且也可以在分子水平上检测到基因组的差异。进一步还要测定单株麦粒的Vc含量,并且对变异材料进行遗传情况评估,以期得到稳定遗传的、新的小麦种质资源。

英文摘要：

By using pollen-tube pathway for introducing the total hot pepper DNA to the wheat, we could obtain the To generation of seeds. After being planted, the total mutation rate of the phenotype of the T1-generation was 21.3%, while that of the control group was 4%. There were 9 kinds of variation spectrum, but it was only 2 in the control group. No matter in the spectrum of variation or the mutation rate, there has obvious differences between the control group and the wheat material after being transformed by the total hot pepper DNA. Randomly selecting the main stem of flag leaf （1/4 of the mucro） of the 47 strains from the transformed materials, we could extract DNA to make IRAP analysis, which showed that totally increased 6 bands, 5 of which existed polymorphism. With pollen-tube pathway introducing the total distant related hot pepper DNA to the wheat, it could cause a variety of variation in the present generation, and it could also detect the difference of genome at the molecular level. To make further research, we also need to determine the vitamin C content of wheat grains for the individual plant, and conduct genetic evaluation for heteromorphosis materials, in order to gain the new stably inherited wheat germplasm resources.