中文摘要：

目的：通过研究mi R-21在慢性阻塞性肺疾病（chronic obstructive pulmonary disease,COPD）患者外周血清及单个核细胞中的表达,探讨mi R-21在COPD发病中的意义及机制。方法：将研究对象分为健康对照组（n=41）和COPD组（n=49）,收集两组外周血清,分离其外周血单个核细胞并收集临床资料。运用实时荧光定量PCR法检测各组外周血清及单个核细胞中mi R-21的表达水平。分析COPD组mi R-21与肺功能的关系,并对mi R-21与COPD患者一秒用力呼气容积（forced expiratory volume in one second,FEV1）进行相关性分析。结果：COPD组外周血清及单个核细胞中mi R-21表达水平较健康对照组增高;COPD外周血清及单个核细胞中mi R-21差异表达与肺功能相关;COPD患者mi R-21表达水平与FEV1呈正相关。结论：COPD患者外周血清及单个核细胞中mi R-21表达升高可能与COPD发病过程及病情严重程度相关。

英文摘要：

Objective： To explore expression of mi R-21 in peripheral blood serum and mononuclear cells of patients with chronic obstructive pulmonary disease（COPD）, and to discuss the signifi cance and underlying mechanisms.Methods： The subjects were divided into a healthy control group（n=41） and a COPD group（n=49）. The mi R-21 level was detected by quantitative real-time PCR. The correlations betweenmi R-21 and lung function or forced expiratory volume in one second（FEV1） were analyzed.Results： The expression levels of mi R-21 in the serum and mononuclear cells in the COPD group were significantly elevated compared with those in the healthy group（P〈0.05）. The expression of mi R-21 was correlated with the lung function of COPD patients. The expression level of mi R-21 in the COPD patients was positively correlated with FEV1. Conclusion： The upregulation of mi R-21 in peripheral blood serum and mononuclear cells of COPD patients may contribute to the pathogenesis of COPD and the severity of this disease.