中文摘要：

目的探讨ERK5（extracellular-regulated kinase 5）MAPK（mitogen-activated protein kinase）信号转导通路与genistein对人乳腺癌细胞MDA-MB-231增殖抑制作用的关系.方法采用噻唑蓝（MTT）比色法检测genistein对MDA-MB-231增殖的抑制作用;采用流式细胞仪检测细胞凋亡情况;应用western blot分别检测ERK5总蛋白和凋亡相关蛋白Bax、Caspase3的表达.结果genistein对MDA-MB-231细胞增殖有明显的抑制作用;流式细胞仪检测到细胞产生凋亡;western blot分析提示genistein抑制ERK5总蛋白的表达,促进Bax和Caspase3蛋白表达.结论genistein可以影响ERK5 MAPK信号转导通路,使凋亡相关蛋白表达增加,抑制MDA-MB-231细胞增殖.

英文摘要：

Objective To study the role of ERK5 MAPK signaling transduction pathway in the inhibition of genistein to MDA-MB-231 breast cancer cells. Methods The inhibitive effects of genistein on MDA-MB-231 cells were observed with the MTT assay. The cell apoptosis was detected by flow cytometry, The ERK5 MAPK, Bax and Caspase3 protein expressions were detected by using western blot method. Results genistein inhibited the cell proliferation of MDA-MB-231 cells and induced apoptosis. The protein expression of ERK5 decreased and those of Bax and Caspase3 increased with the dose of genistein. Conclusion genistein can affect the ERK5 MAPK signaling transduction pathway and induce the expressions of apoptosis related proteins to inhibit the proliferation of MDA-MB-231 cells.