中文摘要：

在玻碳电极表面用电化学沉积法一步合成钻铝水滑石-金纳米粒子（CoAl LDH-GNPs）复合纳米材料,以复合纳米材料作为核酸适配体（Apt）的固定化基质,建立了一种高灵敏的阻抗型适配体传感器。采用扫描电镜（SEM）和能量色散谱仪（EDS）对CoAl LDH-GNPs复合纳米材料进行了表征,对电极的组装过程采用循环伏安法和电化学阻抗进行表征,对传感器的性能采用电化学阻抗进行研究。复合纳米材料CoAl LDHGNPs构筑的传感器对凝血酶（THR）具有良好的信号响应,线性相关系数R=0.995,检出限为0.3 ng/L（S/N=3）,检测范围为1.0 ng/L-100μg/L。

英文摘要：

A highly sensitive impedance aptamer biosensor was fabricated on the glassy carbon electrode surface by one-step electrochemical deposition synthesis of cobalt aluminium layered double hydroxides-gold nanoparticles（CoAl LDH-GNPs） composite nanomaterials,which were used as the immobilization matrix for aptamer.The composite nanomaterials of CoAl LDH-GNPs were characterized with scanning electron microscopy（SEM） and energy dispersive spectrometer（EDS）.The electrode assembly process was monitored by the cyclic voltammetry and electrochemical impedance spectroscopy,the performance of sensor was studied by electrochemical impedance spectroscopy.The sensor fabricated by LDH-GNPs nanomaterials showed a linear relationship to thrombin from 1.0 ng/L to 100.0 μg/L with a linear correlation coefficient R=0.995 and a detection limit of 0.3 ng/L（S/N=3）.