中文摘要：

将树状聚合物（PAMAM）通过共价反应嫁接在羧基化碳纳米管（CNTs）表面后用于钯纳米粒子（Pd NPs）的原位沉积，制备成CNTs-PAMAM/Pd NPs复合物。通过壳聚糖包埋将制备的该钯纳米复合物用于玻碳电极的修饰，并通过交流阻抗法和循环伏安法对电极性能进行表征和优化，制备成可用于葡萄糖的安培检测的非酶电化学传感器。实验表明，在最佳实验条件下该传感器可在较短时间内（＜3 s）实现对葡萄糖的快速稳态安培响应，并可在从0．03 mmol/L至61．7 mmol/L较宽线性范围内实现对葡萄糖的准确测定，检出限为11．5μmol/L。该传感器成本低廉，性质稳定，且具有较好的选择性和重复性，因而在实际应用中具有较好的发展前景。

英文摘要：

Polyamidoamine （PAMAM） dendrimer was covalently grafted with carboxylated carbon nanotubes （CNTs） for the in situ deposition of palladium nanoparticles （Pd NPs）.Based on the electrode modification with the as -prepared CNTs-PAM-AM/Pd NPs nanocomposites and the further coating of chitosan on its surface , a nonenzymatic amperometric glucose biosensor was developed.Electrochemical impedance spectroscopy and cyclic voltammetry were used to characterize and optimize the performance of the sensor.Under the optimal conditions, this sensor exhibited a rapid amperometric response towards glucose （less than 3 s）, and a wide linear range from 0.03 to 61.7 m mol· L^-1 was obtained with a detection limit of 11.5μmol·L^-1 （S/N=3）.This biosensor has low cost , good stability, selectivity and repeatability, thus indicating great potentials for practical applications.