对内蒙古锡盟地区酸马奶中分离出的8株乳酸菌和7株酵母菌进行潜在共生菌的筛选。在各自培养基中交互添加代谢物作为实验组,以各自培养基中添加等量生理盐水作为对照,分析比较稳定期的浊度值。筛选出3对具有相互促进作用的乳酸菌(LC)和酵母菌(YE):LC1和YE3,LC4和YE3,LC5和YE4,其中LC5和YE4相互促进作用极显著(P〈0.01)。LC5和YE4在脱脂乳培养基中共同培养和二者在各自培养基中交互添加代谢物培养都表明其具有相互促进作用,YE4的代谢物促进LC5的生长,使LC5发酵液的滴定酸度值增大,而对pH值变化没有影响;LC5的代谢物促进YE4的生长,使YE4发酵液pH值增大,接近酵母菌最适生长pH值。
A total of 8 lactic acid bacteria(LAB) and 7 yeast strains that had previously been isolated from koumiss from Xilinguole region in Inner Mongolia of China were applied for potential symbiotic characteristic screening.Metabolites of LAB and yeasts were added interactively into their respective media in the treatment groups,while in the control groups the same volume of normal saline was used.The turbidity was monitored during the stationary phase.Three pairs of LAB(LC) and yeast(YE) with positive interaction were disclosed as follows: LC1 and YE3,LC4 and YE3,LC5 and YE4,among which the positive interaction between LC5 and YE4 was highest(P 0.01).LC5 and YE4 co-cultured in skim milk medium showed an increased cell density compared to those obtained in separate culture.The further research showed that the growth of LC5 during fermentation was advanced by the addition of YE4 metabolites with increased titratable acidity and stable pH value.Likewise,the growth of YE4 was also enhanced by LC5 metabolites and the pH value was increased and almost reached the optimum value for yeasts.