位置:成果数据库 > 期刊 > 期刊详情页
锌基-有机金属大环荧光探针对谷胱甘肽生物分子的识别
  • ISSN号:1000-0593
  • 期刊名称:《光谱学与光谱分析》
  • 时间:0
  • 分类:O433.5[机械工程—光学工程;理学—光学;理学—物理]
  • 作者机构:辽宁工业大学化学与环境工程学院,辽宁锦州121001
  • 相关基金:国家自然科学基金项目(21601075); 辽宁省自然科学基金项目(2015020249)资助
作者: 吴红梅, 郭宇
中文摘要:

利用1 H NMR,ESI-MS,UV-Vis,荧光光谱等测试手段研究了基于锌基-有机金属三元大环探针M-1对生物分子谷胱甘肽(GSH)的识别与传感。并且,通过研究识别过程中M-1与组成谷胱甘肽的氨基酸(半胱氨酸、谷氨酸、甘氨酸)的作用关系,确立了M-1对GSH的识别机理。结果表明,化合物M-1在H2O/DMF(1∶9,φ)溶液中形成了稳定的[3+3]大环结构;紫外滴定光谱表明,向M-1中加入GSH后303nm处吸收峰强度增加,380nm处吸收峰强度减弱,在330nm处出现了一个等吸收点,紫外滴定和ESI-MS质谱证实了M-1能够1∶1包合GSH,平衡常数(log KGSH)为4.62±0.15。1 H NMR表明谷胱甘肽在M-1中的构型为组成谷胱甘肽的谷氨酸通过羧基与金属中心之间的静电作用深深地进入M-1空穴内部。荧光光谱表明,向M-1中加入GSH时,以330nm的光激发,发射波长从510nm红移至540nm,荧光强度增加1倍;加入半胱氨酸、谷氨酸时,荧光强度分别增加0.4倍和0.2倍;而加入甘氨酸时,荧光没有变化。综合上述结果证明了M-1空穴的限域作用及其底部三元环上的氨基和GSH上的巯基(半胱氨酸)间的氢键作用使M-1的电子构型发生转变,进而引起紫外光谱和荧光光谱发生变化,实现了大环化合物M-1对生物分子谷胱甘肽的可视化、高灵敏度检测,检测限达到3.0×10-6 mol·L-1。

英文摘要:

Metal-organic supramolecular with well-defined nanosized molecular cavity is able to encapsulate some special guest substrates so that they have been widely applied in molecular recognition and molecular separations.In this work,a highly sensitive recognition of biological molecularγ-glutamyl-cysteinyl-glycine(GSH)was achieved with a trinuclear zinc-based metal-organic cyclohelicate fluorescence probe M-1,which was assembled with zinc ion and two tridentate(N2O)ligand with dansyl sulfonamide group and hydrogen bond site.The recognition process for GSH with M-1was determined with 1 H NMR,ESI-MS,UV-Vis and fluorescence spectra.To establish the recognition mechanism of GSH,its component amino acids(Cys,Glu,Gly)were investigated by spectroscopic titrations.The results show that the M-1with trinuclear structure exhibits high stability in a water/DMF 1∶9solution.UV-Vis adsorption titration revealed that a significant absorbance increase at 303 nm with the addition of GSH to the M-1solution,but an absorbance decrease was observed at 380 nm.A sharp isobestic point was obtained at330 nm.In addition,based on the UV-Vis adsorption titration results,ESI-MS analysis of M-1confirmed that 1∶1complexation stoichiometry of the host-guest behavior was obtained for GSH and the association constant(log KGSH)was calculated as4.62±0.15.Moreover,1 H NMR titrations of M-1upon the above amino acids revealed that the Glu residue of GSH was sent into M-1cavity through the static interactions between the COO-groups and metal ions.In addition,it was found that the fluorescence intensity exhibits a two-fold enhancement,with the emission wavelength red-shifted from 510 to 540nm upon the addition of GSH into M-1.Upon the addition of Cys and Glu in the solution of M-1,the luminescence intensity exhibits 0.4times and 0.2times enhancement,respectively,with the emission wavelength not being shifted.On the contrary,no change of the luminescence intensity was observed after adding the Gly to the solution of M-1.Based on the abov

同期刊论文项目
同项目期刊论文
期刊信息
  • 《光谱学与光谱分析》
  • 中国科技核心期刊
  • 主管单位:中国科学技术协会
  • 主办单位:中国光学学会
  • 主编:高松
  • 地址:北京海淀区魏公村学院南路76号
  • 邮编:100081
  • 邮箱:chngpxygpfx@vip.sina.com
  • 电话:010-62181070
  • 国际标准刊号:ISSN:1000-0593
  • 国内统一刊号:ISSN:11-2200/O4
  • 邮发代号:82-68
  • 获奖情况:
  • 1992年北京出版局编辑质量奖,1996年中国科协优秀科技期刊奖,1997-2000获中国科协择优支持基础性高科技学术期刊奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),荷兰文摘与引文数据库,美国工程索引,美国生物医学检索系统,美国科学引文索引(扩展库),英国科学文摘数据库,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国英国皇家化学学会文摘,中国北大核心期刊(2000版)
  • 被引量:40642