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一种适合于富含多糖和酚类物质的香蕉果实RNA提取方法
  • ISSN号：1009-9980
  • 期刊名称：《果树学报》
  • 时间：0
  • 分类：S668.1[农业科学—果树学;农业科学—园艺学]
  • 作者机构：[1]中国科学院华南植物园,广州510650, [2]华南师范大学生命科学院,广州510631, [3]中国科学院研究生院,北京100039
  • 相关基金：国家自然科学基金重点项目（30430490）.
作者： 刘海[1,2,3], 林德球[2], 徐杰[2], 蒋跃明[1]
关键词： 香蕉, 果实, RNA, 提取, 多糖, 多酚类化合物, Banana, Fruit, RNA, Extraction, Polysaccharides, Polyphenolic compounds
中文摘要：

酚类化合物和多糖是影响香蕉组织RNA提取的几个主要干扰因素。研究通过在裂解液中加入聚乙烯吡咯烷酮去除多酚类化合物和利用水饱和乙醚去除多糖，改进了香蕉果实RNA的提取方法。该方法具有快速、简单等特点。

英文摘要：

Extraction of high-quality RNA from banana fruit pulp tissues is particularly difficult due to high levels of polyphenol and polysaccharides. A suitable method for extracting RNA from banana fruit pulp has been improved by added polyvinylpyrrolidone into the extract solution to remove polyphenolic compounds and using water-saturated ether to remove polysaccharides. In this study, a high-quality RNA from banana fruit pulp tissues was obtained by using the improved method. The procedure was simple and rapid.

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一种适于富含多糖和酚类物质的香蕉果实RNA提取方法(简报)
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期刊信息
  • 《果树学报》
  • 北大核心期刊（2011版）
  • 主管单位:农业部
  • 主办单位:中国农业科学院郑州果树研究所
  • 主编：王志强
  • 地址：河南省郑州市航海东路南中国农业科学院郑州果树研究所
  • 邮编：450009
  • 邮箱：chinagsxb@163.con
  • 电话：0371-65330928 653387308
  • 国际标准刊号：ISSN：1009-9980
  • 国内统一刊号：ISSN：41-1308/S
  • 邮发代号:36-93
  • 获奖情况:
  • 获河南省优秀期刊二等奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘（网络版）,英国农业与生物科学研究中心文摘,美国剑桥科学文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2011版）,中国北大核心期刊（2014版）
  • 被引量:18699