中文摘要：

目的 观察电针对血管性痴呆（VD）大鼠海马组织GluA1与钙离子/钙调素依赖性蛋白激酶Ⅱ（CaMKⅡ）蛋白表达及磷酸化的影响,探讨电针的治疗作用机制.方法 将Wistar大鼠随机分为假手术组、模型组、模拟针刺组和电针组,每组8只.假手术组暴露双侧颈总动脉,但不结扎;模型组、模拟针刺组和电针组采用双侧颈总动脉永久性结扎法建立VD大鼠模型,以新事物识别实验筛选造模成功大鼠.4组大鼠均采用柔软型大鼠固定器固定.电针组选取百会、足三里穴,接通电流,每日1次,每次留针30 min,连续治疗7d;模拟针刺组在百会、足三里穴以毫针刺入皮下0.5 mm;模型组与假手术组只给予固定,不做其它处理.治疗结束后,采用Western Blot法检测大鼠海马组织GluA1、磷酸化GluA1（pGluA1）、CaMKⅡ、磷酸化的CaMKⅡ（pCaMKⅡ）的表达情况,采用Image J图像分析系统对蛋白表达水平进行分析.结果 模型组、模拟电针组、电针组三组大鼠造模后的探索指数[（0.526±0.112）、（0.541 ±0.124）和（0.498±0.099）]均较假手术组（0.785±0.097）明显降低（P＜0.05）;而模型组、模拟针刺组、电针组三组之间两两比较,差异无统计学意义（P＞0.05）.模型组大鼠海马组织GluA1、pGluA1、CaMKⅡ和pCaMKⅡ的表达水平[（1.216 ±0.102）、（1.502 ±0.419）、（1.516±0.392）和（0.394±0.227）]均较假手术组[（1.918±0.137）、（2.253±0.244）、（2.187±0.231）、（0.667±0.175）]明显降低（P＜0.05）.电针组GluA1、pGluA1、CaMKⅡ和pCaMKⅡ的蛋白表达水平[（1.653±0.169）、（2.382±0.308）、（2.733±0.387）、（1.189±0.346）]明显高于模型组和模拟针刺组[（1.231±0.188）、（1.498±0.223）、（1.493±0.205）和（0.408±0.231）],且组间差异均有统计学意义（P＜0.05）.结论 电针可增加VD大鼠海马GluA1及CaMKⅡ的蛋白表达,并促使其发生磷酸化;电针大鼠百会和足三里穴易?

英文摘要：

Objective To observe the effect of electroacupuncture（EA） on the hippocampal expression of GluA1,pGluA1,CaMK Ⅱ and pCaMK Ⅱ in rats with vascular dementia（VD）,so as to find out the underlying mo lecular mechanisms of EA in treating VD.Methods Thirty-two Wistar rats were randomly divided into a shamoperation group,a model group,a sham-acupuncture group,and an EA group （8 in each group）.Permanent bilateral common carotid artery occlusion was performed to model vascular dementia in the model group,the shamacupuncture group and the EA group,while exposure but no occlusion of the bilateral common carotid were performed in the sham-operating group.Novel object recognition test was adopted to prove the establishment of VD rat model.All the rats were kept in an immobilization apparatus while receiving treatments.EA was applied onto ＂ Baihui＂ （GV20） and ＂Zusanli＂ （ST36） in EA group for 30 min,once daily for 7 days.Sham-acupuncture group were treated with needles inserted 0.5 mm superficially.And the sham-operation group and the model group were only immobilized.The protein expression of GluA1,pGluA1,CaMK Ⅱ and pCaMK Ⅱ in hippocampal tissue was detected by western blotting.Results The expression of GluA1 in the model group （1.216 ± 0.102） was significantly less than in the sham-operating group （1.918 ± 0.137） （P 〈 0.05）.The expression of GluA1 in the EA group （1.653 ± 0.169） was significantly higher than in the model group （1.216 ± 0.102） and in sham-acupuncture group （1.231 ±0.188） （P〈0.05）.The expression of CaMKⅡ in the model group （1.516±0.392） was less than in the sham-operating group （2.187 ± 0.231） （P 〈 0.05）.The expression of CaMK Ⅱ in the EA group （2.733 ±0.387） was significantly higher than in the model group （1.516 ±0.392） and sham-acupuncture group （1.493 ±0.205） （P〈0.05）.The expression ofpGluA1 in the model group （1.502 ±0.419） was less than in the sham-operating group （2.253 ± 0.244） （P