中文摘要：

目的评价富氢液对大鼠慢性炎性痛的影响。方法健康雄性SD大鼠32只，8～10周龄，体重200～250g，采用随机数字表法，将其分为4组（n=8）：对照组（C组）、完全弗氏佐剂组（CFA组）、富氢液组（H2组）和完全弗氏佐剂＋富氢液组（CFA＋H2组）。CFA组和CFA＋H2组采用左后肢足底注射完全弗氏佐剂100μl的方法制备大鼠慢性炎性痛模型。H2组和CFA＋H2组于造模后1d开始腹腔注射0．6mmol／L富氢液5ml／kg，1次，d，连续7d；其余2组腹腔注射等容量生理盐水。分别于造模前1d和造模后1、3、7d时，测定机械缩足反应阈（MWT）和热缩足潜伏期（TWL）；于造模后7d痛阈测定结束后处死大鼠，取L4-5，节段脊髓组织，采用Westernblot检测核因子E2相关因子2（Nrf2）和血红素氧合酶-1（HO-1）的表达。结果与C组比较，H，组MWT、TwL和脊髓组织Nrf2和HO-1的表达差异无统计学意义（P〉0．05），CFA组和CFA＋H2组造模后各时点MWT降低，TWL缩短，脊髓组织Nrf2和HO-1的表达上调（P〈0．05）；与CFA组比较，CFA＋H2组造模后各时点MWT升高，TWL延长，脊髓组织Nrf2和HO-1的表达上调（P〈0．05）。结论 富氢液可减轻大鼠慢性炎性痛，其机制与激活脊髓Nrf2／ARE信号通路有关。

英文摘要：

Objective To evaluate the effects of hydrogen-rich saline on chronic inflammatory pain in rats. Methods Thirty-two male Sprague-Dawley rats, aged 8-10 weeks, weighing 200-250 g, were randomly divided into 4 groups （ n = 8 each） using a random number table： control group （group C） ; complete Freund＇s adjuvant （CFA） group; hydrogen-rich saline group （group H2）; CFA ＋ hydrogen- rich saline group （CFA＋H2 group）. Chronic inflammatory pain was induced by injecting CFA 100μl into the plantar surface of the left hindpaw in CFA and CFA ＋ H2 groups. In H2 and CFA ＋ H2 groups, 0.6 mmol/L hydrogen-rich saline 5 ml/kg was injected intraperitoneally once a day for 7 consecutive days starting from 1 day after injection of CFA, while the equal volume of normal saline was given instead of hydrogen-rich saline in C and CFA groups. The mechanical paw withdrawal threshold （MWT） and thermal paw withdrawal latency （TWL） were measured at 1 day before CFA injection and 1, 3 and 7 days after CFA injection. The rats were sacrificed after the last measurement of pain threshold on day 7 after CFA injection. The left lumbar segments （L4-5 ） of the spinal cord were removed for determination of nuclear factor E2-related factor 2 （Nrf2） and heme oxygenase-1 （HO-1） expression by Western blot. Results Compared with C group, no significant change was found in the MWT, TWL and expression of Nrf2 and I40-1 in 1t2 group, and the MWT was significantly decreased, the TWL was shortened, and the expression of Nrf2 and HO-1 was up-regulated in CFA and CFA＋H2 groups. Compared with CFA group, the MWT was significantly increased, the TWL was prolonged, and the expression of NrlE and HO-1 was up-regulated in CFA＋H2 group. Conclusion Hydrogen-rich saline can alleviate chronic inflammatory pain in rats, and activation of Nrf2/ARE signaling pathway in the spinal cord is involved in the mechanism.