中文摘要：

研究采用螯合剂及超滤技术脱除岩藻聚糖中重金属元素的方法。在提取岩藻聚糖工艺中添加不同浓度的EDTA二钠和植酸溶液,对所制备的岩藻聚糖采用电感耦合等离子体-质谱技术（ICP-MS）及氢化物-原子荧光光谱法（HGAFS）检测其Mg、Ca、Fe、Mn、Cu、Zn、Ag、Cd、Ba、Pb、As、Hg元素的含量。研究表明,在提取工艺中加入1.0×10-2mol/LEDTA二钠可以有效地降低岩藻聚糖中Mg、Ca、Mn、Zn、As、Ag、Cd、Pb等元素的残留量,但没有减少Hg、Fe、Cu、Ba元素的残留量;而提取工艺中加入0.10mol/L植酸仅对减少Ag、Cd和Zn元素的残留量有一定的效果。通过对岩藻聚糖进行As、Hg富集处理,研究在酸处理后采用超滤脱除重金属元素的效果,结果表明,各实验组均未检出As元素,但对Hg元素没有效果。因此,在提取岩藻聚糖的工艺中添加一定浓度的EDTA二钠或在纯化过程中经酸处理后采用超滤脱除技术均可显著减少岩藻聚糖制品中重金属元素的残留量,从而制备高质量的岩藻聚糖。

英文摘要：

In order to remove heavy metals,different concentrations of ethylene diamine tetraacetic acid disodium （EDTA-Na2） and fytic acid were separately added during the extraction of fucoidans from brown seaseeds,and the prepared products were determined by inductively coupled plasma-mass spectrometry （ICP-MS） and hydride generation atomic fluorescence spectroscopy （HGAFS） for the contents of metal elements （such as Mg,Ca,Fe,Mn,Cu,Zn,Ag,Cd,Ba,Pb,As and Hg）. Adding 1.0 × 10-2 mol/L EDTA-Na2 could efficiently reduce the contents of Mg,Ca,Mn,Zn,As,Ag,Cd and Pb in the prepared fucoidans,but had no effect on Hg,Fe,Cu and Ba. However,only Ag,Cd and Zn exhibited lower contents due to the addition of 0.10 mol/L fytic acid. The simultaneous binding of As and Hg to fucoidans was conducted in order to evaluate the efficiency of removing heave metal element by acidic treatment followed by ultrafiltration. As a result,no As was detected in the ultrafiltration retenate,while Hg was kept bound with fucoidans after acidic treatment and could not be removed. Thus,adding an appreciate concentration of EDTA-Na2 during extraction and acidic treatment followed by ultrafiltration during purification can obviously reduce the contents of heavy metal elements in the prepared fucoidans. This study provides a good strategy for preparing high-quality fucoidans.