中文摘要：

目的：通过超高压（HHP）对柯萨奇病毒B（cBV）毒力和病毒免疫原性的影响，探讨既能降低病毒毒力又能保留病毒免疫原性的有效方法，为制备CBV疫苗提供新的途径。方法：病毒毒力检测采用50％组织细胞感染量试验（TCIDso）法；病毒免疫原性检测采用巨噬细胞吞噬功能测定、抗体检测、T淋巴细胞转化实验。结果：压力达到680MPa时，病毒毒力降低，病毒TCID50由10^5上升为10^-4高于700MPa时，病毒被灭活。对病毒进行加热、压力减毒及压力灭活，小鼠巨噬细胞吞噬功能、抗体产生量及T淋巴细胞转化率与盐水对照组比较差异有显著性（P〈0．05），加热组、减毒组与灭活组之间比较差异无显著性（P〉0．05）。结论：CB3V对压力的抵抗力强，灭活CB3V的临界压力为700MPa；压力灭活的CB3V免疫原性不受影响。

英文摘要：

Objective To evaluate the influences of high hydrostatic pressure （HHP） on the virulence and antigenicity of Coxsackie virus Ba （CBaV） , to explore a new physiological method to develop an active vaccine against Coxsackie virus, Methods The phagocytosis of the macrophages in mice to the CBa V and the TCIDs0 of the CBaV before and after treated by HHP were examined. Results TCID50 increased from 10^-5 to 10^-4 when treated by 680 MPa; viruses were inactivated at 700 MPa. Viruses were also inactivated by the pressure less than 700 MPa, but needed prolonged time. The phagocytic function, antibody and T lymphocyte transformation rate in experimental mice （heated, attenuated, and inactive viruses） exhibited a significant difference compared with control mice （P（ 0.05）. Conclusion CB3V has a strong resistance to HHP. The inactivated pressure is about 700 MPa, however, the antigenicity of the virus is not influenced by the same value of HHP.