欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
从长沙岳麓山和湘江等地富含油脂的样品中分离得到一株产脂肪酶菌株CS 1-1,经18S rDNA序列分析,确定该菌株为黑曲霉（Aspergillus niger）.对该菌株的产酶条件进行优化,发现该菌株在以体积分数为1%的橄榄油为诱导物,5 g/L蔗糖为碳源,40 g/L蛋白胨为有机氮源,1 g/L（NH4）2SO4为无机氮源,pH为6.5的培养基中,于28℃,180 r/min摇床培养48 h,可达最大酶产量（37.6±0.8）mmol.min-1.L-1.
英文摘要：
Strain CS 1-1 was isolated from the rich oil sample of Yuelu mountain and Xiangjiang river in Changsha city,which was identified as Aspergillus niger based on 18S rDNA sequence analysis.The fermentation conditions for lipase production by strain CS 1-1 were optimized,and the maximum lipase production of（37.6±0.8） mmol·min·L-1 was obtained at 28 ℃,180 r/min and pH 6.5 after 48 h Incubation using olive oil（1%） and sucrose（5 g/L） as combination of peptone and carbon sources,yeast extract（40 g/L） and ammonium sulfate（1 g/L） as nitrogen sources.
同期刊论文项目
同项目期刊论文
Single and cooperative bioleaching of sphalerite by two kinds of bacteria - Acidithiobacillus ferrio
Preservation of Acidithiobacillus caldus: A moderately thermophilic bacterium and the effect on subs
Isolation of Phosphate-Solubilizing Fungi from Phosphate Mines and Their Effect on Wheat Seedling Gr
Large-scale Hydrothermal Synthesis and Characterization of Size-controlled Lanthanum Hydroxide Nanor
Identification and fermentation optimization of protopectinase-overproducing strain Aspergillus nige
Bioleaching of chalcopyrite concentrate by a moderately thermophilic culture in a stirred tank react
The effect of preferential flow on extraction and surface morphology of copper sulphides during heap
Comparative genomic analysis of Acidithiobacillus ferrooxidans strains using the A. ferrooxidans ATC
Isolation and identification of moderately thermophilic acidophilic iron-oxidizing bacterium and its
Characterization and Reconstitute of a [Fe4S4] Adenosine 5 '-Phosphosulfate Reductase from Acidithio
Comparison of microbial communities in three different mine drainages and their bioleaching efficien
Effects of samarium dopant on photocatalytic activity of TiO2 nanocrystallite for methylene blue deg
Simulated small-scale pilot plant heap leaching of low-grade oxide zinc ore with integrated selectiv
Hydrothermal synthesis and electrochemical properties of alpha-manganese sulfide submicrocrystals as
Expression, purification and characterization of the sulfite reductase hemo-subunit, SiR-HP, from Ac
Optimization of separation processing of copper and iron of dump bioleaching solution by Lix 984N in
Glu43 is an essential residue for coordinating the [Fe2S2] cluster of IscR from Acidithiobacillus fe
In vitro assembly of [Fe4S4] cluster in high potential iron-sulfur protein from Acidithiobacillus fe
Expression, purification and characterization of a high potential iron-sulfur protein from Acidithio
Bioleaching of sphalerite by Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans cultur
期刊信息
位置：