中文摘要：

目的：观察镇肝熄风汤对1-甲基-4-苯基-1，2,3，6-四氢吡啶（1-methy-4-phenyl-1，2，3，6．tetrahy-dropyridine，MPTP）诱导的C57BL／6小鼠黑质神经元凋亡的保护作用及其可能机制。方法：C57BL／6小鼠皮下注射MPTP（30mg／kg）制备帕金森病小鼠模型，灌胃给予镇肝熄风汤预处理。运用爬杆试验检测小鼠的运动协调能力，采用AnnexinV-FITC双染法测定细胞凋亡率，实时定量RT-PCR法检测α-突触核蛋白（α-Syn）mRNA表达，分光光度法检测caspase-3酶活性。结果：与模型组比较，镇肝熄风汤可显著缩短帕金森病小鼠爬杆时间（P〈0．05），抑制黑质多巴胺能神经元凋亡（P〈0．05），下调α-SynmRNA表达，减少caspase-3酶活性（P〈0．05）。结论：镇肝熄风汤具有抗实验性帕金森病的作用，其机制可能与抑制α-Syn介导的黑质多巴胺能神经元凋亡有关。

英文摘要：

Objective： To investigate the protective effect of Zhengan Xifeng Decoction on dopaminergic neurons in the substantia nigra from toxicity of MPTP in C57BL/6 mice, and some possible involved mechanisms. Methods： C57BL/6 mice were given MPTP （30mg/kg） to prepare parkinson disease mice model. Different doses of Zhengan Xifeng Decoction （12.5, 25, 50g/kg）, or selegiline （10mg/kg） were given 10 days prior to MPTP in the pretreatment groups. Climbing pole test was used to estimate the movement coordination of mice. Apoptosis of neurons were determined by Annexin V-FITC/PI staining. Real-time PCR was used to analyze the expression of α-synuclein mRNA. Caspase-3 activity in the brain was determined by spectrophotometry. Results： Compared with model group, Zhengan Xifeng Decoction could diminish average time of climbing pole in MPTP-treated C57BL/6 mice in climbing pole test （P〈0.05）; Zhengan Xifeng Decoction could also protect against neurons apoptosis in the substantia nigra （P〈0.05）, down-regulate the expression of α-synuclein mRNA, and decrease caspase-3 activation （P〈0.05）. Conclusion： Zhengan Xifeng Decoction exerted anti-parkinson disease effect in mice via depression apoptosis of dopaminergic neurons in the substantia nigra, and this effect might be attributable to regulation mRNA expression of α-synuclein.