中文摘要：

老鼠胚胎的干细胞(mESCs ) 源于培植前胚胎的内部房间质量(ICM ) ，当有教养时，能在与 GSK3 禁止者 CHIR99021 和 MEK 禁止者 PD0325901 补充的 N2B27 媒介维持无差别的状态(“ 2i') 并且白血病禁止者因素(LIF ) 。比作常规文化媒介，这媒介的所有部件被定义。与 N2B27 媒介， 2i 和 LIF，然而， mESCs 能贡献妄想的胚胎的 germline all-ES 房间老鼠是否能被 tetraploid 互补产生，还是不清楚的，当时 tetraploid 互补用作一个金色的标准估计 ES 房间的 pluripotency。这里，我们的学习证明 mESCs 发源并且与 N2B27 完全的媒介有教养能由 tetraploid 互补产生肥沃的老鼠。另外，生来的 mES 房间线生产的 tetraploid 互补老鼠的幸存率比常规文化条件高，并且增加积极房间对比到常规中等任何一个的 Oct4 的百分比。因此，与 2i 和 LIF 补充的 N2B27 媒介是为推导和老鼠的长期的文化的一种其他的选择胚胎的干细胞。

英文摘要：

Mouse embryonic stem cells （mESCs） derived from inner cell mass （ICM） of preimplantation embryos, can maintain undifferentiated state when cultured in N2B27 medium supplemented with GSK3 inhibitor CHIR99021 and MEK inhibitor PD0325901 （“2i”） and leukemia inhibitor factor （L1F）. Compare to conventional culture medium, all components of this medium are defined. With the N2B27 medium, “2i” and LIF, mESCs can contribute to the germline of the chimeric embryos, however, whether the “all-ES cells” mice can been generated by tetraploid complementation is unclear yet, while the tetraploid complementation serve as a golden standard to assess the pluripotency of ES cells. Here, our study showed that mESCs derived and cultured with the N2B27 complete medium could generate fertile mice by tetraploid complementation. In addition, the survival rate of tetraploid complementation mice produced by inbred mES cell lines is higher than the conventional culture condition, and increased the percentage of Oct4 positive cells contrast to conventional medium either. Therefore, the N2B27 medium supplemented with “2i” and LIF is an alternative choice for the derivation and longterm culture of mouse embryonic stem cells.