中文摘要：

-thymosins, 高度保存的肽的一个家庭，起一个重要作用在愈合创伤， angiogenesis，抗菌剂过程和抗病毒的免疫。三新奇 -thymosin-repeat 蛋白质，命名 mjthm4， mjthm3 和 mjthm2，从抑制用表示顺序标签(EST ) 从 Marsupenaeus japonicus 被克隆减少性的杂交。拥有的 mjthm4， mjthm3 和 mjthm2 cDNAs 打开编码了 166， 128 和 90 的读的框架氨基酸残余多肽并且包含了四，三和二 -thymosin 肌动朊绑定模块分别地。强风分析证明 mjthm4， mjthm3 和 mjthm2 与已知的无脊椎的多重复 -thymosins 分享了高相同。这些蛋白质无所不在地检验纸巾在所有被表示，并且 transcriptional 层次在肠是最高的。进一步的调查表明 mjthm4， mjthm3 和 mjthm2 是显著地起来调整的在 WSSV 感染以后的 6 h。而且当 mjthm4 transcriptional 层次没显示变化时， mjthm3 和 mjthm2 层次在病毒抵抗的虾减少了。结果显示 mjthm4， mjthm3 和 mjthm2 是新奇多重复 -thymosin 相当或相同事物，与 WSSV 感染有一种靠近的关系，并且可能贡献在虾的主人防卫或病毒侵略相互作用的更好的理解。

英文摘要：

β-thymosins, a family of highly conserved peptides, play a vital role in wound-healing, angiogenesis,antimicrobial process and antiviral immunity. Three novel β-thymosin-repeat proteins, named mjthm4, mjthm3 and mjthm2, were cloned from Marsupenaeus japonicus using expressed sequence tags（EST） from suppression subtractive hybridization. The mjthm4, mjthm3 and mjthm2 c DNAs possessed open reading frames that encoded166, 128 and 90 amino acid residue polypeptides and contained four, three and two β-thymosin actin binding modules, respectively. Blast analysis demonstrated that mjthm4, mjthm3 and mjthm2 shared high homology with known invertebrate multi-repeat β-thymosins. These proteins are ubiquitously expressed in all of the examined tissues, and the transcriptional levels were highest in the intestine. Further investigation revealed that mjthm4,mjthm3 and mjthm2 were remarkably up-regulated 6 h after WSSV infection. Moreover, while mjthm4 transcriptional levels displayed no changes, mjthm3 and mjthm2 levels decreased in the virus-resistant shrimps.The results indicate that mjthm4, mjthm3 and mjthm2 are novel multi-repeat β-thymosin homologues, have a close relationship with WSSV infection, and might contribute to a better understanding of host defense and/or virus invasion interactions in shrimps.