中文摘要：

采用常规的用于测定活细胞个数的试剂2-（2-甲氧基-4-硝苯基）-3-（4-硝苯基）-5-（2,4-二磺基苯）-2H-四唑单钠盐（WST-8四唑盐）建立了一种快速测定细胞活性的表面增强拉曼散射光谱（SERS）新方法.考察了p H值及还原剂烟酰胺腺嘌噙二核苷酸（NADH）用量对WST-8甲瓒信号的影响.结果表明,与传统的WST-8比色法相比,SERS法具有更高的检测灵敏度和选择性,对WST-8甲瓒的检测限可达到10μmol/L.

英文摘要：

Surface-enhanced Raman scattering（ SERS） technique was used to develop and optimize a novel and quantitative 2-（ 2-methoxy-4-nitrophenyl）-3-（ 4-nitrophenyl）-5-（ 2,4-disulfophenyl）-2H-tetrazolium monosodium salt（ WST-8） assay for living cell viability. The influences of p H and dosage of reductant nicotinamide adenine dinucleotide（ NADH） on WST-8 formazan signal were examined. SERS signals of formazan with high resolution and great diversity between WST-8 and formazan were observed in neutral conditions.Meanwhile,the signals of formazan（ 560,1351,1598,1616 cm-1） could be found clearly when the concentration of NADH was four times that of WST-8. Compared with the traditional method,this assay appears higher sensitivity and selectivity for WST-8 formazan. Moreover,the detection limit of formazan is as low as 10μmol /L.