中文摘要：

目的探讨Th1／Th2亚群失衡在大鼠深静脉血栓形成（DVT）中的作用及中药复方消栓通脉颗粒逆转Th1／Th2亚群失衡治疗DVT的作用机制。方法80只DVT大鼠随机分为消栓通脉颗粒组、DVT组、假手术组、正常组，分离大鼠脾脏单个核细胞，流式细胞术检测各组大鼠Thl亚群（CD4＋TNF—α）、Th2亚群（CD4＋IL-4＋）比例，ELISA法检测大鼠外周血TNF—α、IL-4水平。结果与正常组及假手术组比较，DVT大鼠脾脏Th1亚群比例显著升高（P=0．022），Th2亚群比例显著降低（P=0．015）；血清TNF-α水平显著升高（P=0．005）；IL-4水平显著降低（P=0．012）。消栓通脉颗粒作用后，大鼠脾脏Thl亚群比例显著降低（P=0．008），Th2亚群比例显著升高（P=0．011），血清TNF-α水平降低（P=0．036），IL-4水平升高（P=0．018）。结论Th1／Th2亚群失衡参与了大鼠DVT发病过程，消栓通脉颗粒诱导T细胞向Th2亚群偏移，抑制炎性因子表达进而减轻炎症反应，保护血管内皮细胞，促进血栓再通。

英文摘要：

Objective To evaluate the role of Th1/Th2 shift in deep vein thrombasis （DVT） and elucidate the mechanism of Traditional Chinese Medicine （TCM） -Xiaoshuantongmai Decoction-in treating DVT animal models. Methods 40 DVT rats were divided randomLy in to DVT group and TCM group.Additionally, 20 rats with sham operation were taken as sham operation group and 20 normal rats were taken as normal group respectively. Rats of TCM group were treated with Xiaoshuantongmai Decoction for 8-15 days,while rats of other groups were treated with double distilled water for the same duration. Then,the proportions of Thl （CD4＋TNFα ＋） subset and Th2 （CD4＋IL-4＋） subset were detected by Floweytometry. Moreover,the protein expressions of TNF-α and IL-4 in rat serum were analyzed by ELISA assay. Results Compared with normal and sham operation group, the proportion of Thl subset was increased while that of Th2 subset was decreased significantly in DVT group. In addition,protein expression of TNF-α was enhanced while that of IL-4 was inhibited markedly in DVT rats. After TCM administration,the proportion of Thl subset was down-regulated while that of Th2 subset was up-regulated significantly in DVT rats. Moreover,the protein expression of TNF- α decreased while that of IL-4 increased obviously after TCM administration. Conclusion Thl/ Th2 shift was involved in DVT.TCM- Xiaoshuantongmai decoction-down-regulated the Thl subsets whereas upregulated the Th2 subsets;thereby, Xiaoshuantongmai decoction exerted the curative effect on DVT mice.