中文摘要：

这份报纸试图开发 monoclonal 的目的抗体(MAbs )- 为检测 Chlamydophila pneumoniae 的基于的 ELISA (C。pneumoniae ) 在有可变领域(VD ) 的人的抗原 2 专业和 3 外部膜蛋白质(MOMPVD2-VD3 ) 并且认出 C 的基本身体由与对有能力的广泛地使用的 MAb 作比较估计它的敏感和特性。pneumoniae。方法 MOMPVD2-VD3 是在 Escherichia coli 的 overexpressed 并且由亲密关系层析净化了。老鼠与 recombinant 抗原被使免疫，并且 hybridomas secreting MAbs 被屏蔽。三稳定的 hybridomas 克隆被选择并且称为 5D6， 7G3，和 8C9。基于 MAbs 的 ELISA 从组与很多件人的喉咙拖把样品为种类特定的识别被细看我(有以前临床上证实的典型的呼吸的病的 156 个病人) 并且组 II (57 个健康施主) 。在组的结果我， 55 个积极盒子被 anti-EB 检测基于 MAb 的 ELISA， 51 个盒子由 MAbs 5D6 底是积极的 ELISA，和 33 和 38 个盒子分别地由 MAb 8C9 和 7G3 底 ELISA 是积极的。从组 II 健康施主的 57 件样品， 5 是积极的， 52 与 anti-EB 和 5D6 底测试是否定的，当 2 和 3 个积极盒子被另外的二基于 MAb 的 ELISA 分别地识别时。新奇 MOMPVD2-VD3 基于 MAb 的试金可以比 anti-EB MAb 有更高的特性的结论，它可以可能为 C 的诊断被用作一个其他的工具。pneumoniae 感染。

英文摘要：

Objective This paper aims to develop a monoclonal antibodies （MAbs）- based ELISA for detecting Chlamydophila pneumoniae （C. pneumonioe） antigens in humans with the variable domains （VD） 2 and 3 of the major outer membrane protein （MOMPvD2-VD~） and to assess its sensitivity and specificity by comparing with a widely used MAb that is able to recognize the elementary bodies of C. pneumoniae. Methods MOMPvo2-vo3were overexpressed in Escherichia coil and purified by affinity chromatography. Mice were immunized with the recombinant antigen, and hybridomas secreting MAbs were screened. Three stable hybridomas clones were selected and named 5D6, 7G3, and 8C9. The MAbs-based ELISA was scrutinized for species-specific recognition with a number of human throat swab samples from Group I （156 patients with typical respiratory illness clinically confirmed before） and Group II （57 healthy donors）. Results In Group I, 55 positive cases were detected by anti-EB MAb-based ELISA, 51 cases were positive by MAbs 5D6-based ELISA, and 33 and 38 cases were positive by MAb 8C9 and 7G3-based ELISA respectively. Of the 57 samples from Group II ＂healthy donors＂, 5 were positive and 52 were negative with both anti-EB and 5D6-based tests, while 2 and 3 positive cases were identified by the other two MAb-based ELISAs respectively. Conclusion The novel MOMPvD2.VD3 MAb-based assay may have higher specificity than the anti-EB MAb, which may possibly be used as an alternative tool for the diagnosis of C. pneumoniae infection.