中文摘要：

采用电子克隆与RT-PCR相结合的技术，在条锈菌（Pucciniastriiformis f．sp．tritici）侵染的小麦中克隆了一个LSDl型锌指蛋白基因，命名为TaLSDl（GenBank登录号为EF553327）。序列分析表明，该基因全长1024bp，编码生成1个包含3个保守LSDl型锌指结构（CxxCxRxxLMYxxGASxVxCxxC）且长度为146个氨基酸的多肽。进化树分析表明，TaLSDl与水稻（Oryzasativa）、拟南芥（Arabidopsisthaliana）和芜菁（Brassicarapa）中部分含有3个保守LSDl型锌指结构的同源基因亲缘关系较近，而与其它包含不同数目的LSDl型锌指结构基因亲缘关系较远。推测TaLSDl在进化中丢失了部分序列，进而执行新的功能。半定量RT-PCR结果显示，该基因在亲和以及非亲和组合中的表达模式很相似，均表现在前期基因表达被抑制而后期恢复正常。初步推测TaLSDl在转录水平上的表达受光诱导，同时，作为一个细胞程序性死亡的负调控因子在小麦与条锈菌互作过程中起作用。

英文摘要：

Using in silico cloning and RT-PCR,an LSD1 type zinc finger protein gene, TaLSD1 ,was cloned from wheat infected by stripe rust fungus （Puccinia striiformis f. sp. tritici）. Sequence analysis showed that the full-length cDNA of TaLSD1 was 1 024 bp,which encoded a polypeptide of 146 amino acids with three conserved motifs （CxxCxRxxLMYxxGASxVxCxxC）. Phylogenetic tree indicated that TaLSD1 might share a common ancestor with other LSD1 type zinc finger protein genes which contained three con- served LSD1 type zinc finger motifs in rice （Oryza sativa） ,turnip （Brassica rapa） and Arabidopsis （Ara- bidopsis thaliana） ,whereas it had a further relationship with other homologous genes with different num- ber of LSD1 type zinc finger motifs. It was assumed that TaLSD1 performs new function since some infor- mation had been lost during evolution. The results of semi-quantitative RT-PCR showed that the transcrip- tion profile of TaLSD1 ,which became normal after being down-regulated in the early stage,was similar inincompatible interaction and compatible interactions. It was speculated that the transcription of TaLSD1 was light-induced and TaLSD1 function as a negative regulator of programmed cell death （PCD） in the in- teraction of stripe rust fungus and wheat.