中文摘要：

目的探索应用CpG寡脱氧核苷酸刺激对慢性淋巴细胞白血病（CLL）细胞染色体分析的影响。方法对70例CLL患者的外周血（或骨髓）标本，分别应用植物血凝素、CpG寡脱氧核苷酸和CpG寡脱氧核苷酸联合IL-2刺激培养72h，常规收获细胞制备染色体并进行R显带分析。应用组合探针进行间期细胞荧光原位杂交检测。结果植物血凝素组、CpG寡脱氧核苷酸组和CpG寡脱氧核苷酸联合IL-2组染色体分析成功率（可分析分裂象≥20个）分别为90．0％、68．6％和68．6％，异常核型检出率分别为3．2％、43．6％和43．6％，组合探针荧光原位杂交异常核型检出率为64．3％。结论应用CpG寡脱氧核苷酸刺激培养CLL细胞，可提高染色体异常检出率。

英文摘要：

Objective To explore the effect of CpG-oligodeoxynucleotides （ODN） in chromosome study of chronic lymphocytic leukemia （CLL）. Methods Blood or bone marrow cells of 70 CLL patients were cultured for 72 h with PHA , CpG-ODN and CpG-ODN combined with IL-2, respectively. Routine karyotype analysis with R banding technique and interphase fluorescence in situ hybridization （FISH） were performed. Results The metaphase number ≥ 20 was considered as successful stimulation, which in PHA, CpG-ODN and CpG-ODN combined IL-2 groups were 90.0% , 68.6% and 68.6%, respectively, and the detection rates of chromosome aberrations were 3.2% , 43.6% and 43.6% , respectively. The aberrations rates detected by interphase FISH with a panel of probes was 64.3%. Conclusion CpG-ODN DSP30 can effectively raise the detection rate of chromosome aberrations in CLL patients.