中文摘要：

[目的]利用cDNA-AFLP技术对耐盐性受主效基因控制的小麦品系98-160盐胁迫前后基因表达的变化进行分析。[方法]对Il2个克隆的cDNA差异表达片段进行了B1ast-x分析。[结果]约有65．2％的片段与已知基因有较高的同源性，从中筛选出18个与小麦耐盐性密切相关的片段，主要包括转录因子、与离子转运有关的蛋白、信号传导相关蛋白、胁迫相关蛋白以及与蛋白-蛋白相互作用有关的蛋白。以94号片段为基础，通过电子延伸和RT-PCR，克隆了小麦液泡膜ATPaseC亚基（TaVHA～C）的全长cDNA，Northern结果表明，在胁迫的早期（0～2h）TaVHA-C基因的表达基本不变，随着胁迫时间的延长（6～12h），乃VIIA-C基因的表达逐渐减弱，以后（24～72h）又逐渐增强。低温对TaVHA-C基因的表达有明显的抑制作用。[结论]利用cDNA-AFLP技术分离差异表达片段进行相关基因的研究是可行的。

英文摘要：

[Objective] 98-160 is a wheat line with excellent salt-tolerance that is controlled by major genes. The expression profiles of 98-160 stressed by salt for different times were studied via cDNA-AFLP. [Method] One hundred and twelve differentially expressing cDNA fragments were obtained, which were sequenced and analyzed by Blast-x. [Result] One hundred and twelve fragments were cloned and sequenced. The Blastx analysis showed that 62.5% of the sequenced fragments were similar to known genes in the database, in which 18 cDNA were tightly related to wheat salt tolerance, most of them were transcription factor, transportation related protein, signal transduction related protein and proteins related to other stresses. Some fragments encoded proteins involved in protein-protein interaction. Based on a cDNA fragment similar to vacular ATPase C subunit, full-length cDNA of a vacular ATPase C subunit in wheat （TaVHA-C） was obtained using electric PCR and RT-PCR. Northern blot analysis indicated that drought and salt stress had a similar effect on the expression of TaVHA-C. The expression of TaVHA-C was steady at the early stage of stress （0 to 2 hours）, it decreased subsequently （6 to 12 hours） and increased in the end （ 24 to 72 hours）. Significant inhibition was observed of low temperature on the expression of TaVHA-C. [Conclusion] These results further proved that it was viable to isolate genes of plants with large genome size via cDNA-AFLP.