中文摘要：

目的：研究海参岩藻聚糖硫酸酯（ SC-FUC）对巨噬细胞的调节作用及相关信号通路，探究其调节机体免疫的作用机制。方法噻唑蓝（ MTT）比色法检测巨噬细胞的增殖情况；中性红法检测吞噬活性；Griess试剂法测定培养上清液中NO含量；RT -PCR检测巨噬细胞下游细胞因子IL-6、IL-10、Toll样受体和相关信号分子MyD88、TRIF、NF-κB的mR-NA表达量。结果 SC-FUC 对巨噬细胞增殖、吞噬能力和NO分泌均有促进作用，在作用前期（6h和12h）效果更为明显。 SC-FUC能上调细胞因子 IL-6和 IL-10的 mRNA 表达量，上调 TLR4、TLR5、TLR9的表达量，促进信号分子MyD88、TRIF和NF-κB的mRNA表达量。结论 SC-FUC能够激活巨噬细胞，促进其分泌NO、IL-6、IL-10等细胞因子，从而发挥免疫调节作用。 SC-FUC激活巨噬细胞的信号通路与细胞表面TLR4、TLR5、TLR9及NF-κB通路相关。

英文摘要：

Aim To investigate the immunomodulatory effects of sea cucumber fucoidan （ SC-FUC） on macro-phage and the signaling pathways. Methods Cell via-bilities in response to different concentrations of SC-FUC were analyzed by MTT, phagocytosis ability was detected by neutral red,and nitric oxide （ NO） produc-tion was examined by Griess reaction kit. The mRNA expression levels of IL-6 , IL-10 , Toll-like receptors （TLRs） and related signal molecules MyD88, TRIF, NF-κB were assayed by real-time PCR. All the experi-ments were based on murine RAW264. 7 cell line. Re-sults SC-FUC could promote RAW264 . 7 cell prolif-eration, phagocytosis as evidenced by uptake of neutral＆amp;nbsp;red and release of NO. The effects were significant at the early stage （6 h and 12 h） . SC-FUC could up-reg-ulate the expression of IL-6 , IL-10 , TLR4 , TLR5 , TLR9. Moreover, mRNA expressions of TLRs signaling molecules were increased, as well as MyD88, TRIF, NF-κB. Conclusions SC-FUC could activate macro-phage, and then promote the immune function by pro-moting production or expression of NO, IL-6, IL-10. It is speculated to be relevant to activated cell surface re-ceptors in macrophage, including TLR4, TLR5, TLR9, and NF-κB signaling pathways.