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基于荧光素标记的DNA-Cu（Ⅱ）络合物的荧光增强型氰根离子传感器

ISSN号：0567-7351
期刊名称：《化学学报》
时间：0
分类：O614.121[理学—无机化学;理学—化学]
作者机构：[1]f中国科学院化学研究所有机周体实验审、北京市海淀区中关村北一街2号,北京100190
相关基金：项目受国家自然科学基金（Nos.21021091,21075126）、科技部973计划项目（No.2011CB808400）以及电子科技大学电子薄膜与集成器件固家重点实验室开放基金（No.KFJJ201010）资助.

作者：黄显虹[1], 张关心[1], 张德清[1]

关键词：氰根离子, 寡核苷酸, 铜离子, 荧光传感器, cyanide, oligo DNA, copper ion, fluorescence sensor

中文摘要：

利用铜离子与荧光素标记的寡核苷酸链、氰根离子结合能力的差异, 发展了一种水相中氰根离子检测的新方法。研究表明, 该检测方法可以较灵敏地检测水相中的氰根离子, 其检测下限达到0.02 μmol/L, 且具有较好的选择性和潜在的实际应用价值。

英文摘要：

In this paper, a new fluorescence turn-on sensor for cyanide in aqueous solution was established with fluo- rescein-labeled oligo DNA. The design rationale is explained as follows：（1） Fluorescein-labeled oligoDNA （FAM-DNA） shows strong fluorescence in aqueous solution. But, it is expected that the fluorescence of FAM-DNA will be quenched be- cause of the formation of stable complex of FAM-DNA with Cu^2＋; （2） Demetallation from the complex of FAM-DNA with Cu^2＋ because of the reaction of CN and Cu^2＋ and formation Cu（CN）2 ; as a result the fluorescence of FAM-DNA emerges again. Therefore, it is anticipated that the fluorescence of the ensemble of FAM-DNA and Cu^2＋ increases by in- creasing the concentration ofCN . Thus, the ensemble of FAM-DNA and Cu^2＋ can be utilized for the detection ofCN . The results revealed that the fluorescence of FAM-DNA were quenched efficiently in the presence of Cu^2＋（1.35 μmol/L） due to the intramolecular photoinduced electron transfer within the ensemble of FAM-DNA and Cu^2＋. Other metal ions, such as K^＋, Ca^2＋, Ba^2＋, Mg^2＋, Fe^3＋, Hg^2＋, Ni^2＋, Pb^2＋, Zn^2＋ and Ag^＋, induced negligible fluorescence quenching for FAM-DNA under the same conditions. However, fluorescence of the system increased gradually by increasing the concentration of CN-in the ensemble solution, and the fluorescence intensity varies almost linearly vs. the concentration of CN . The detection limit of CN- with the ensemble of FAM-DNA and Cu^2＋ was estimated to be 0.02 μmol/L, which is much lower than the maximum level （1.9 μmol/L） of CN- in drinking water permitted by the World Health Organization （WHO）. Moreover, the response of the ensemble of FAM-DNA and Cu^2＋ to solutions containing CN- （40 μmol/L） in the presence of relevant ionic species, including F^-, CI^-, Br^-, I^-, NO3^-, HSO4^-,CO3^2-, CH3COO^-, H2PO4^-, SCN^-, and N3^- （at a concentration level over 10 times higher to that of CN-）, was investigated. The results indicate