中文摘要：

目的：建立分离天然HMGN2的方法并研究其抗茵性与对中性粒细胞的趋化活性。方法：5％高氯酸对子宫纤维囊腺瘤组织进行萃取，萃取物通过反相高效液相色谱（RP—HPLC）直接进行分离，Tdcine-SDS-PAGE，AU-PAGE和Western-Blot对蛋白分子进行鉴定，采用Transwell实验检测HMGN2对中性粒细胞的趋化性。结果：分离出高纯度的HMGN2，HMGN2对大肠杆菌有杀菌活性但HMGN2在体外不能趋化中性粒细胞运动。结论：建立简单、直接分离高纯度的天然HMGN2的方法，有助于对其进一步研究，我们发现HMGN2对大肠杆菌有杀菌活性但它不能通过趋化中性粒细胞发挥作用。

英文摘要：

Objective： To establish a method for separating and purifying HMGN2 and to study the antibacterial activity of HMGN2 and its chemotaxis to neutrophil. Methods： We extract the uterus fiber Cystadenoma with 5% perchlorate and separate HMGN2 from the extracts by reversed - phase high performance liquid chromatography （RP-HPLC） directly. By tricine- SDS-PAGE, AU-PAGE and Western-Blot, we identify the protein as HMNG2, and we research antibacterial activity of HMGN2 through agarose gel diffusion assays and study its chemotaxis to human neutrophil by chemotaxis assay. Results： We have got high purified HMGN2 ,which have antibacterial activity to E. colt but in vitro can not make neutrophil migrate. Conclusion：We find a simple and direct way of separating and purifying nature HMGN2,which will be useful for the research of HMGN2. we discover that HMGN2 have antibacterial activity against E. colt but HMGN2 can not make neutrophil migrate.