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中文摘要：
目的探讨PCR-ELISA法及荧光定量PCR法（FQ-PCR）检测HBV感染者精液中HBV-DNA检出情况及其临床意义。方法选取男性HBV感染者60例,采集患者精液、静脉血,用PCR-ELISA法来检测患者精液及血清中HBV-DNA。同时采用荧光定量PCR检测患者精液中HBV-DNA。结果PCR-ELISA法：60例精液标本HBVDNA阳性12例,阳性率18.33%,拷贝数为1.267×10^3-6.532×10^5/ml,平均拷贝数为4.781×10^4/ml;60例血清标本阳性53例,阳性率为88.33%,拷贝数1.462×10^3-5.153×10^7/ml,平均拷贝数为2.451×10^6/ml。荧光定量PCR法：60例精液标本HBVDNA阳性13例,阳性率21.67%,拷贝数为1.357×10^3-7.113×10^5/ml,平均拷贝数为4.983×10^4/ml;两种检验方法检测精液HBV-DNA的阳性检出率比较无显著性意义（P〉0.05）。结论PCR-ELISA法与荧光定量PCR定量检测精液中HBVDNA同样具有较强的特异性和灵敏度,为临床了解乙型肝炎患者精液中肝炎病毒的感染状态提供了帮助,具有重要的临床意义。
英文摘要：
Objective To evaluate the value of PCR - ELISA and FQ - PCR to test HBV DNA in HBV sufferers＇semens. Methods HBV DNA in 60 HBV sufferers＇ semens and serums were tested with quantitative PCR - ELISA and FQ - PCR. Results Using PCR - ELISA, there were 12 positive samples of HBV DNA in 60 semen samples, and the positive was 18.33%. The copies were 1. 267 ×10^3 - 6.532 ×10^5/ml, and the average copy was 4.781 ×10^4/ml. There were 53 positive samples of HBV DNA in 60 serums samples, and the positive is 88.33%. The copies were 1.462 ×10^5 -5. 153 ×10^7/ml, and the average copy is 2.451 ×10^6/ml. Using FQ - PCR, there were 13 positive samples of HBV DNA in 60 semen samples, and the positive was 21.67%. The copies were 1. 357 ×10^3 -7. 113 ×10^5/ml, and the average copy was 4. 983 ×10^4/ml. There are no difference between PCR- ELISA and FQ- PCR. Conclusion PCR -ELISA quantitative test and FQ -PCR have similar speciality and sensitivity, which has important clinical significance to evaluate the situation of HBV DNA viruses in semen samples.
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