中文摘要：

目的本实验设计合成靶向核因子KB（NF-KB）的哑铃形圈套寡核苷酸（ODNs），并验证其对白介素10（IL-10）的作用。方法以阳离子脂质体为载体，将圈套ODNs转染大鼠pMФ细胞6h后用LPS（μg／ml）刺激，用ELISA法及RT-PCR法测圈套ODNs对IL-10在蛋白及mRNA水平的影响。结果哑铃形圈套ODNs可降低在IL-10mRNA及蛋白水平的表达。结论靶向NF-KB的哑铃形圈套ODNs具有抗IL-10的作用。

英文摘要：

Objective： Decoy - ODNs in dumbbell shape with the nuclear sequence similar to NF - kB cis - elements were synthetized in this study. To measure the anti -inflammatory action of decoy -ODNs targeting NF - kB. Methods： With carriers of cationic liposomes, decoy ODNs were transfected into pMqb cells from rats. Then the inhibiting effects of the decoy - ODNs of IL - 10 were analysized. Results Decoy - ODNs could decrease the expression of IL - 10 . Conclusions ： Decoy - ODNs targeting nuclear factor - kB can decress the expression of IL - 10 in pMФ ceils.