中文摘要：

目的：通过研究铜锌超氧化物歧化酶（Cu/Zn superoxide dismutase，Cu/Zn SOD或SOD1）的蛋白表达和活性变化，探讨川芎嗪（tetramethylplyrazine，TMP）拮抗庆大霉素（Gentamycin，GM）耳毒性作用的抗氧化机制。方法将60只听力正常豚鼠随机分为GM组、TMP组、GM＋TMP组和对照组4个组，每组15只。GM组肌注硫酸庆大霉素120 mg·kg-1· d-1，TMP组腹腔注射盐酸川芎嗪30 mg·kg^-1·d^-1，GM＋TMP组肌注硫酸庆大霉素120 mg·kg^-1·d^-1及腹腔注射盐酸川芎嗪30 mg·kg^-1·d^-1，对照组腹腔注射与GM组等量的生理盐水2.5 ml·kg^-1·d^-1；每组连续注射12天。每组首次用药前及末次用药后行ABR检测。实验结束后取耳蜗标本，将耳蜗组织研磨，采用Western blot法检测SOD1蛋白表达，ELISA法检测SOD1活性。结果用药后GM＋TMP组ABR反应阈值明显低于GM组（P〈0.01），但较对照组、TMP组仍有升高；GM组、GM＋TMP组SOD1蛋白表达水平高于TMP组、对照组（P〈0.05），且GM＋TMP组高于GM组（P〈0.05）；SOD1活性强弱顺序为：GM＋TMP组〉对照组≈TMP组〉GM组。结论川芎嗪可能通过调控SOD1的蛋白表达及活性变化降低庆大霉素的耳毒性，推测这是川芎嗪抗氧化作用机制之一。

英文摘要：

Objective To observe the effects of the expression and activity of Cu/Zn superoxide dismutase （Cu/Zn SOD or SOD1） on antagonistic action of TMP on Gentamycin-induced hearing loss.Methods 60 healthy guinea pigs were divided randomly into four groups： model group （GM group）, therapy group （GM＋TMP group）, TMP group and normal control group, 15 in each group. GM group received intramuscular injection of Gentamycin sulfate 120 mg·kg^-1·d^-1 for 12 days. TMP group received TMP 30 mg·kg^-1·d^-1 intraperitoneal injection for 12 days. GM＋TMP group received intramuscular injection of Gentamycin sulfate 120 mg·kg^-1·d^-1, intraperitoneal injection of TMP 30 mg·kg^-1·d^-1 for 12 days. The normal control group was administered with daily intramuscular injection of saline 2.5 ml·kg^-1·d^-1 for 12 days. Auditory brainstem responses （ABR） were used before and after the animals were tested,Western blot was used to test protein expression of SOD1 and ELISA to test SOD1 activity.Results Before treatment ABR thresholds in each group showed no significant difference （P〉0.05）;after treatment, GM group＆#39;s ABR thresholds were significantly higher than GM＋TMP group （P〈0.01）, TMP group and the normal control group＆#39;s ABR thresholds before and after treatment did not change significantly （P〉0.05）. The protein expression of SOD1 increased significantly in GM＋TMP group and GM group, and GM＋TMP group was higher than that in GM group （P〈0.05）. The order of SOD1 activity in the cochlear tissue：GM＋TMP group〉 normal control group≈ TMP group〉 GM group.Conclusion TMP can produce the anti-oxidant effects through adjust the protein expression and activity of SOD1.