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中文摘要：
用硼氢化钠还原法制备了金硒（AuSe）纳米合金。用单链核酸适配体（aptamer）修饰AuSe纳米合金制备了汞离子的核酸适配体纳米探针（Apta-AuSe）。在pH 6.8 Na2HPO4-NaH2PO4缓冲溶液中及NaCl存在下,Apta-AuSe纳米探针亦不聚集;当Hg2＋存在时,它可以稳定aptamer序列中的T-T错配,形成较稳定的双链T-Hg2＋-T错配物,从而使释放出来的AuSe纳米合金聚集形成较大的微粒,导致590 nm处共振散射峰增强。Hg2＋浓度在1.3~1 466 nmol.L-1范围内与共振散射光强度呈良好线性关系,其回归方程和检出限分别为ΔI590 nm=0.603c＋2.0和0.74 nmol.L-1。该方法用于水样中Hg2＋的检测,结果满意。
英文摘要：
Under the condition of sodium citrate as stabilizer,the gold-selenium（AuSe） nano-alloy was prepared by sodium borohydride reduction procedure,and was modified by single-strand aptamer to obtain an aptamer nano-alloy probe（apta-AuSe） for Hg（Ⅱ）.In pH 6.8 Na2HPO4-NaH2PO4 buffer solution and in the presence of NaCl of 33 mmol·L-1,the Apta-AuSe probe is not aggregation.The apta-AuSe interacts with Hg2＋ to form stable double-strand T-Hg（Ⅱ）-T mismatches and to release AuSe nano-alloy particles from the probe.The released AuSe nano-alloy particles（20∶1） aggregated to form bigger clusters that resulted in the resonance scattering（RS） intensity（I590 nm） increasing at 590 nm.The increased intensity ΔI590 nm was proportional to the Hg2＋ concentration from 1.3 to 1 466 nmol·L-1,with a detection limit of 0.74 nmol·L-1.The regress equation was ΔI590 nm=0.603c＋2.0.Thus,a new resonance scattering（RS） spectroscopy of apta-AuSe was applied to the analysis of trace mercury ion.This simple,rapid,selective and sensitive aptamer AuSe nano-alloy RS assay was applied to the determination of Hg2＋ in wastewater,with satisfactory results.
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