中文摘要：

目的：观察电针双侧＂扶突＂等穴对甲状腺区切口痛大鼠颈段脊髓中5-羟色胺（HT）受体（R）亚型5-HT 1AR和5-HT 2AR mRNA及蛋白表达的影响,探讨电针缓解颈部切口痛的可能机制。方法：Wistar雄性大鼠随机分为正常组、模型组、扶突组、合谷-内关组、足三里-阳陵泉颈段组、足三里-阳陵泉腰段组,每组8只。沿大鼠颈中线做长约1.5cm的纵行切口,制作切口痛模型。分别电针双侧＂扶突＂＂合谷＂-＂内关＂＂足三里＂-＂阳陵泉＂穴,各30min。用辐射热测大鼠切口部位热痛阈;用荧光定量Real-time PCR和免疫印迹（Western blot）方法检测颈段（C1-C4）、腰段（L1-L3）脊髓5-HT 1A和5-HT 2A受体亚型mRNA和蛋白的表达水平变化。结果：与颈部切口术前比较,各组的热辐射躲避潜伏期明显缩短（P〈0.05）;电针＂扶突＂穴或＂合谷＂-＂内关＂穴后,与本组术后比较其躲避潜伏期显著延长（P〈0.05）;电针＂足三里＂-＂阳陵泉＂与本组术后比较差异无统计学意义（P〉0.05）。与正常组比较,模型组颈段脊髓中5-HT 1AR mRNA的表达明显升高（P〈0.05）,5-HT 2AR mRNA和蛋白的表达均明显上调（P〈0.05）;与模型组比较,扶突组和合谷-内关组脊髓中5-HT 1AR的mRNA表达水平均明显下降（P〈0.05）,5-HT 2AR的mRNA和蛋白的表达水平均明显上调（P〈0.05）,足三里-阳陵泉组5-HT 1AR和5-HT 2AR的mRNA表达水平与模型组比较差异均无统计学意义（P〉0.05）。结论：电针＂扶突＂和＂合谷＂-＂内关＂穴可缓解大鼠颈部急性切口痛,该作用可能与其下调颈段脊髓中5-HT 1AR mRNA表达、上调5-HT 2AR mRNA和蛋白的表达水平相关。

英文摘要：

Objective To observe the effect of electroacupuncture（EA） at bilateral ＂Futu＂（LI18）,etc.on the expression of 5-HT1A receptor（R） mRNA,5-HT2AR mRNA and protein in the spinal cord of rats with neck incision pain,so as to explore its underlying mechanism in relieving incision pain.Methods A total of 48 Wistar rats were randomly divided into control,model（incision pain）,Futu（LI18）,Hegu（LI4）-Neiguan（PC6,LI4-PC6）,Zusanli（ST36）-Yanglingquan（GB34） cervical cord（ST36-GB34 C） and lumbar cord（ST36-GB34 L） groups.A 1.5cm longitudinal incision was made in the middle of the neck under Isoflurane inhalation anesthesia.Pain threshold（PT） was measured using radiant heat.EA（1-2mA,2Hz/15Hz） was applied to bilateral LI18,LI4-PC6,ST36-GB 34 for 30min.The expression of 5-HT1AR mRNA,5-HT2AR mRNA and protein in the cervical spinal cord（C1-C4） tissue,and 5-HT1AR mRNA and 5-HT2AR mRNA in the lumbar cord（L1-L3） were detected with Real-time PCR and Western blot analysis,respectively.Results After the operation,the thermal PT was shortened obviously in comparison with pre-operation（P0.05）.Compared with post-operation,the PT values were increased markedly in the LI18 group and LI4-PC 6 group（P0.05）,rather than in the ST36-GB 34 group（P0.05）.The expression levels of 5-HT1AR mRNA,5-HT2AR mRNA and 5-HT2AR protein in the cervical cord of the model group were increased significantly compared with those of the control group（P0.05）.In comparison with the model group,the expression level of 5-HT1AR mRNA was down-regulated considerably in the LI18 and LI4-PC 6 groups（P0.05）,and those of spinal 5-HT2AR mRNA and protein were up-regulated significantly in the LI18 and LI4-PC 6 groups（P0.05）.No significant changes were found in the expression of 5-HT1AR mRNA and 5-HT2AR mRNA in the ST36-GB 34 C group in comparison with the model group（P0.05）.The expression levels of 5-HT1AR mRNA and 5-HT2AR mRNA in the ST36-GB34 L group were significantly lower than those of the ST3