欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
本实验室前期研究发现，2型糖尿病动物模型ob／ob小鼠血清中miR-122的含量较正常C57BL／6小鼠显著升高．本文进一步研究肝脏特异性miR-122及其靶蛋白AldoA（果糖1，6-二磷酸醛缩酶A）在ob／ob小鼠肝脏代谢中的作用．首先，经qRT-PCR技术检测发现ob／ob小鼠肝脏miR．122水平较C57BL／6小鼠显著下降，而Westernblotting分析发现ob／ob小鼠肝脏其靶蛋白AldoA的表达水平显著上升．进一步以miR-122分子转染293T细胞后收集其分泌的微囊泡（microvesicles，Mrs），经qRT-PCR检测确认后采用特异性荧光染料DiI-C18标记MWs，以不同剂量尾静脉注射BALB／c小鼠体内，不同时间点取肝组织做冰冻切片．在荧光显微镜下观察证实，包裹有miR．122的MVs通过循环系统进入肝脏，同时qRT-PCR定量分析发现肝组织中miR-122含量显著升高，而蛋白质印迹检测发现其靶蛋白AldoA在肝脏中表达显著下降．AldoA主要催化糖酵解途径中果糖1，6-二磷酸和磷酸二羟丙酮及甘油醛-3-磷酸之间的转变，miR-122靶向作用AldoA可能在2型糖尿病的发生发展中发挥重要作用．
英文摘要：
In previous study, our group have found that there is a significant increased level of miR-122 in the serum of ob/ob mice, an animal model of type 2 diabetes. Here, we further investigated the role of miR-122 targeting AldoA in the liver ofob/ob mice. First, a significant decrease ofmiR-122 level and a notable increase of AldoA expression was found in the liver of the ob/ob mice. Second, mature miR-122 was transfected into 293T cells and then MVs isolated from 293T cells were collected; qRT-PCR was applied to confirm that miR-122 was rich in MVs. Third, specific fluorescent dye DiI-Cls-labeled MVs were injected intravenously into BALB/c mice; the frozen section of liver was observed through fluorescent microscopy. Finally, miR-122 targeting AldoA in the metabolism of ob/ob mice was confirmed by qRT-PCR and Western blotting. AldoA mainly catalysed the transformation between dihydroxyacetone phosphate, glyceraldehyde-3- phosphate and fructose 1, 6 - bisphosphate in glycolytic pathway. MiR-122 may play an important role in the pathologenesis of oh/oh mice through AldoA pathway.
同期刊论文项目
同项目期刊论文
Synthesis, biological evaluation, 3D-QSAR studies of novel aryl-2H-pyrazole derivatives as telomeras
Identification and characterization of microRNAs in the crab- eating macaque (Macaca fascicularis) u
Design, synthesis and antibacterial activity studies of thiazole derivatives as potent ecKAS III inh
Serum microRNAs profile from genom-wide serves as a fingerprint for diagnosis of acute myocardial in
Synthesis, biological evaluation, and molecular docking studies of novel 1,3,4-oxadiazole derivative
Application of an Endophytic Bacillus amyloliquefaciens CC09 in Field Control of Rehmannia glutinosa
Ultrasensitive detection of lead ion based on target induced assembly of DNAzyme modified gold nanop
Mitochondrial Inhibitor Sensitizes Non-Small-Cell Lung Carcinoma Cells to TRAIL-Induced Apoptosis by
Molecular Docking Explores the Possible Binding mode for Potential Inhibitors of Thioredoxin Glutath
Quercetin and allopurinol reduce liver thioredoxin-interacting protein to alleviate inflammation and
Vaticaf？nol, a resveratrol tetramer, exerts more preferable immunosuppressive activity than its prec
Design, modi？cation and 3D QSAR studies of novel naphthalin-containing pyrazoline derivatives with/w
Synthesis, biological evaluation and molecular modeling studies of Schiff bases derived from 4-methy
Betaine recovers hypothalamic neural injury by inhibiting astrogliosis and inflammation in fructose-
Twoconserved oligosaccharyltransferase catalytic subunits required forN-glycosylation exist in Spart
Cloning of novel rice blast resistance genes from two rapidly evolvingNBS-LRR gene families in rice.
Widely distributed hot and cold spots in meiotic recombination as shown by sequencing of rice F2 pla
Reactive oxygen species-induced TXNIP drives fructose-mediated hepatic inflammation and lipid accumu
Wuling San protects kidneydysfunction by inhibiting renal TLR4/MyD88 signaling and NLRP3 inflammasom
Enhanced charge transfer by gold nanoparticle at DNA modified electrode and its application to label
Widely distributed hot and cold spots in meiotic recombination as shown by the sequencing of rice F-
Bacterial community structure in cooling water and biofilm in an industrial recirculating cooling wa
Desertification dynamic and the relative roles of climate change and human activities in desertifica
Design, synthesis andbiological evaluation of novel pyrazolinecontaining derivatives as potentialtub
Dihydropyrazoles containingmorpholine: design, synthesis and bioassay testing aspotent antimicrobial
Sulfonamide derivativescontaining dihydropyrazole moieties selectively and potently inhibitMMP-2/MMP
Simple electrochemicalsensing of attomolar protein using fabricated complexes with enhanced surfaceb
Mitochondrial InhibitorSensitizes Non-Small-Cell Lung Carcinoma Cells to TRAIL-Induced Apoptosis byR
The evolution of soybeanmosaic virus: An updated analysis by obtaining 18 new genomic sequences ofCh
Pterostilbene and allopurinol reduce fructose-induced podocyte oxidative stress and inflammation via
EGFR/HER-2 inhibitors:synthesis, biological evaluation and 3D-QSAR analysisof dihydropyridine-contai
Design, synthesis,biological evaluation and molecular modeling of dihydropyrazole sulfonamidederivat
Identification of four serum microRNAs from a genome-wide serum microRNA expression profile as poten
期刊信息
位置：