内部核糖体入口地点(怒火) 广泛地在遗传工程被使用了;然而,在蚕(Bombyx mori ) 的申请几乎没被报导。在这研究, Encephalomyocarditis 的部分顺序的生物活动 EMCV 和 RhPV 的怒火的病毒(EMCV ) 怒火, Rhopalosiphum padi 病毒(RhPV ) 怒火,和混血儿在 Spodoptera frugiperda (Sf9 ) 被调查房间线和蚕纸巾。EMCV 和 RhPV 的混合怒火在在昆虫和蚕支持下游的基因表示显示出比 EMCV 怒火或 RhPV 怒火有效的更多。在蚕的中间的丝绸腺的所有 IRES 的活动比在胖身体和以后的丝绸腺的那些高。EMCV 和 RhPV 的混合怒火集成于由转基因的技术的蚕染色体测试怒火的生物活动。每积极转基因的个人有报告基因 EGFP 的重要表示。这些结果建议怒火有一个潜力在蚕的遗传工程研究被过去常。
Internal ribosome entry site (IRES) has been widely used in genetic engineering; however, the application in silkworm (Bombyx morl) has hardly been reported. In this study, the bio-logical activity of partial sequence of Encephalomyocarditis virus (EMCV) IRES, Rhopalosiphum padi virus (RhPV) IRES, and the hybrid of IRES of EMCV and RhPV were investigated in Spodoptera frugiperda (Sf9) cell line and silkworm tissues. The hybrid IRES of EMCV and RhPV showed more effective than EMCV IRES or RhPV IRES in promoting downstream gene expression in insect and silk- worm. The activities of all IRESs in middle silk gland of silkworm were higher than those in the fat body and pos- terior silk gland. The hybrid IRES of EMCV and RhPV was integrated into silkworm genome by transgenic tech-nology to test biological activity of IRES. Each of the posi- tive transgenic individuals had significant expression of report gene EGFP. These results suggested that IRES has a potential to be used in the genetic engineering research of silkworm.