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The relationship between internal domain sequences ofpiggyBac and its transposition efficiency in BmN ceils and Bombyx mori
  • ISSN号:1672-9145
  • 期刊名称:《生物化学与生物物理学报:英文版》
  • 时间:0
  • 分类:S852.65[农业科学—基础兽医学;农业科学—兽医学;农业科学—畜牧兽医] Q78[生物学—分子生物学]
  • 作者机构:[1]College of Animal Sciences, Zhejiang University, Hangzhou 310029, China, [2]Institute of Sericulture, Chengde Medical College, Chengde 067000, China, [3]College of Environmental & Resource Sciences, Zhejiang University, Hangzhou 310029, China, [4]College of Animal Sciences, South China ofAgriculturalUniversity, Guangzhou 510642, China
  • 相关基金:This work was supported by the grants from the National Basic Research Program of China (No. 2012CB114601) and the National Natural Science Foundation of China (No. 30972142).
中文摘要:

内部核糖体入口地点(怒火) 广泛地在遗传工程被使用了;然而,在蚕(Bombyx mori ) 的申请几乎没被报导。在这研究, Encephalomyocarditis 的部分顺序的生物活动 EMCV 和 RhPV 的怒火的病毒(EMCV ) 怒火, Rhopalosiphum padi 病毒(RhPV ) 怒火,和混血儿在 Spodoptera frugiperda (Sf9 ) 被调查房间线和蚕纸巾。EMCV 和 RhPV 的混合怒火在在昆虫和蚕支持下游的基因表示显示出比 EMCV 怒火或 RhPV 怒火有效的更多。在蚕的中间的丝绸腺的所有 IRES 的活动比在胖身体和以后的丝绸腺的那些高。EMCV 和 RhPV 的混合怒火集成于由转基因的技术的蚕染色体测试怒火的生物活动。每积极转基因的个人有报告基因 EGFP 的重要表示。这些结果建议怒火有一个潜力在蚕的遗传工程研究被过去常。

英文摘要:

Internal ribosome entry site (IRES) has been widely used in genetic engineering; however, the application in silkworm (Bombyx morl) has hardly been reported. In this study, the bio-logical activity of partial sequence of Encephalomyocarditis virus (EMCV) IRES, Rhopalosiphum padi virus (RhPV) IRES, and the hybrid of IRES of EMCV and RhPV were investigated in Spodoptera frugiperda (Sf9) cell line and silkworm tissues. The hybrid IRES of EMCV and RhPV showed more effective than EMCV IRES or RhPV IRES in promoting downstream gene expression in insect and silk- worm. The activities of all IRESs in middle silk gland of silkworm were higher than those in the fat body and pos- terior silk gland. The hybrid IRES of EMCV and RhPV was integrated into silkworm genome by transgenic tech-nology to test biological activity of IRES. Each of the posi- tive transgenic individuals had significant expression of report gene EGFP. These results suggested that IRES has a potential to be used in the genetic engineering research of silkworm.

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期刊信息
  • 《生物化学与生物物理学报:英文版》
  • 北大核心期刊(2004版)
  • 主管单位:
  • 主办单位:中国科学院上海生物化学研究所
  • 主编:
  • 地址:上海岳阳路319号
  • 邮编:200031
  • 邮箱:abbs@sibs.ac.cn
  • 电话:021-54920956 54920955
  • 国际标准刊号:ISSN:1672-9145
  • 国内统一刊号:ISSN:31-1940/Q
  • 邮发代号:4-210
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,荷兰文摘与引文数据库,美国生物医学检索系统,美国剑桥科学文摘,美国科学引文索引(扩展库),美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),英国英国皇家化学学会文摘,中国北大核心期刊(2000版)
  • 被引量:5851