中文摘要：

运用PCR和RT．PCR技术从萝卜中克隆出风膪”基因，其cDNA包含一个长度为1038bp的开放阅读框，编码345个氨基酸，蛋白质相对分子质量为36．37×103。TMHMMServerv2．0分析RslRTl蛋白有8个跨膜结构域，推测为一种膜蛋白；蛋白序列同源分析表明，萝卜RslRTl（AFJ05595）与拟南芥AtlRTl（XP一002869968）、遏蓝菜TclRTl一P（CAL25151）的遗传距离最近。半定量RT—PCR表达分析表明，正常铁营养时凡m丌基因在萝卜花瓣、花蕾、叶片和根中均不表达，缺铁胁迫及缺铁加镉（100mg·L-1）胁迫下表达，且缺铁加镉胁迫下，叶片和根中RslRTl的表达量均高于缺铁胁迫。研究结果表明凰儡＂基因受外源缺铁和镉胁迫所诱导，凰侬¨参与金属铁和镉的吸收和转运过程。

英文摘要：

In this study, a RsIRT1 gene was isolated from radish with PCR and RT-PCR techniques, and the cDNA sequence contained a complete open reading frame（ORF） of 1 038 bp that encodes 34：; amino acids with a molecular weight of 36.37 x 103. The hydrophobieity analysis based on TMHMM Server v2.0 found that RsIRT1 had eight putative transmembrane domains （TM）and it might be localized in the plasma membrane. DNAman analysis revealed that RsIRT1 （ AFJ05595 ） shared high homology with TcIRT1-P （CAL25151）and AtIRT1 （XP-002869968）. Moreover, semi-quantitative RT-PCR showed that the RsIRT1 gene were expressed in petal,bud,leaf and root under Fe-deficiency and Fe-deficiency with Cd（ 100 mg L-1） conditions. The RslRTI gene showed higher expression profiles in leaves and roots under Fe-deficieney with Cd conditions compared with Fe-defieiency conditions, whereas no expression of RslRT1 was observed under normal Fe condition. These results suggested that RsIRT1 gene could be induced under Fe-defieiency and Fe-deficiency with Cd stresses, and it mi~ght be involved in the process of Fe and Cd uptake and transport in radish.