中文摘要：

为探讨山羊子宫内膜细胞和性腺激素对子宫自然杀伤（uNK）细胞分泌血管内皮生长因子（VEGF）和y-干扰素（IFN-y）含量的影响。本研究以永生化山羊子宫内膜基质细胞（ESC）和上皮细胞（EEC）为体外研究模型，通过ELISA法检测子宫内膜细胞和性腺激素（E2和P4）对uNK细胞分泌VEGF和IFN-y含量的影响。结果显示，在uNK细胞与EEC共培养时，雌激素Ez单独或与P4共同作用可显著抑制uNK细胞中IFN-y和VEGF分泌水平（P〈0．05），而P4单独作用不显著；而当uNK细胞与ESC共培养时，孕酮P4单独或与E。共同作用可显著增强IFN-y在uNK细胞中的分泌水平（P〈O．05），而E2单独或与P4共同作用可显著抑制VEGF分泌水平（P〈O．05）。研究结果表明性腺激素对与EEC或ESC共培养的uNK细胞分泌水平具有不同程度的调节作用。

英文摘要：

To explore the effects of goat endometrial ceils and sex hormones on secretion content of VEGF and IFN-y in uNK cells. Using immortalized goat endometrial stromal cells （ESC） and epithelial cells （EEC） as an in vitro research model, the effects of the endometrial cells and sex hormones （E2, P4 ） on secretion content of VEGF and IFN-y, in uNK cells was detected by ELISA assay. When uNK cells co-cultured with EEC, estrogen alone or together with P4 significantly in- hibited the secretion levels of IFN-7 and VEGF in uNK cells （P〈0.05）, while the P4 alone had no significant effect. When uNK cells co-cultured with ESC, P4 alone or together with E2 can sig- nificantly enhance the secretion of IFN-y, in uNK cells （P〈0.05）, whereas E2 alone or together with P4 significantly inhibited the secretion level of VEGF （P〈0.05）. These results indicated that the sex hormones play a role in regulatoring secretion of uNK cells co-cultured with EEC or ESC.