中文摘要：

以筛选得到的鲍曼不动杆菌（Acinetobacter baumannii）基因组DNA为模板，PCR扩增了磷酸烯醇式丙酮酸羧化酶（PEPC）基因，构建了克隆载体pET·28a—PEPC。将测序结果正确的重组子转化到大肠杆菌BL21中，获得重组菌BL21-pET-28a—PEPC。经IPTG诱导，该重组菌实现了PEPC的高效表达，并且生长速度明显快于对照菌大肠杆菌BL21，可作为宿主用于构建碳固定工程菌。

英文摘要：

The phosphoenolpyruvate carboxylase （PEPC） gene was amplified via the polymerase chain reaction （PCR） from Acinetobacter baurnannii, and an inducible expression vector pET-28a-PEPC was constructed and transformed into E. coli BL21. And the recombinant strain named BL21-pET-28a-PEPC has been obtained. Sodium dodecyl sulfate-polyaerylamide gel electrophoresis （SDS-PAGE） analysis showed a high expression of PEPC in the host. Notably, the engineered E. coli grew faster than the control, which indicates its applicability as a host for an engineering strain for carbon fixation.