中文摘要：

目的：观察活血化瘀中药秃毛冬青叶中有效成分青心酮对RAW264．7细胞炎症反应时可溶性TOU样受体4mRNA及蛋白表达、血红素氧和酶1mRNA表达、肿瘤坏死因子d分泌的影响。 方法：①实验于2004-03／2005-01在华中科技大学同济医学院病理生理教研室完成。②采用RAW264．7巨噬细胞株建立细胞炎症反应模型。观察下述4种情况下培养液血红素氧和酶1mRNA的变化：脂多糖刺激；青心酮[青心酮注射液，3，4-羟基苯乙酮40mg／支（2mL），实验用，北京制药三厂]预处理3h后加脂多糖刺激；青心酮和脂多糖同时加入；脂多糖刺激3h后加入青心酮。其他指标测定实验分为3组：空白对照组（正常细胞培养，加入等数量的培养基，不加入任何药物）；青心酮预处理组（先加入溶有青心酮1×10^-7mol／L的培养基培养3h，再加入脂多糖10μg/L刺激4，8h）；未处理组（加入等数量的培养基3h，再加入脂多糖10μg/L刺激4，8h）。③采用反转录聚合酶链反应分析可溶性TOU样受体4、血红素氧和酶1mRNA的变化；Western blot方法检测可溶性Toll样受体4蛋白水平的改变，用ELISA方法检测培养液肿瘤坏死因子α分泌的变化。④组间数据处理采用配对t检验。 结果：①可溶性TOU样受体4mRNA表达：青心酮预处理脂多糖刺激4h后明显高于未处理组（P〈0．01）。未处理组经脂多糖刺激4h后，明显低于空白对照组（P〈0．05）。②可溶性TOU样受体4蛋白表达：青心酮预处理组脂多糖刺激8h后明显高于未处理组（P〈0.05），但与空白对照组比较，差异不明显（P〉0．05）；未处理组脂多糖刺激8h后，明显低于空白对照组（P〈0，05）。③血红素氧和酶1mRNA表达：青心酮预处理后加脂多糖刺激和青心酮、脂多糖同时加入后明显高于脂多糖刺激后（P〈0．05）。④培养液中肿瘤坏死因子d：脂多糖刺激后明显增加（P〈0?

英文摘要：

AIM： To observe the effect of dihydroxyacetone （DHAP）, which is the effective component of holly leaves and can promote blood circulation by removing blood stasis, on the expressions of soluble Toll-like receptor 4 （sTLR4） mRNA and protein, expression of hemeoxygenase-1 （HO-1） mRNA and the secretion of tumor necrosis factor alpha （TNF-α） in the inflammatory reaction of RAW264.7 cell lines. METHODS：① The experiment was finished in the Department of Pathophysiology, Tongii Medical College, Huazhong University of Science and Technology from March 2004 to January 2005. ②The RAW264.7 cell lines were used to establish the models of inflammatory reaction. The changes of HO-1 mRNA were observed in the following conditions： lipopolysaccharide （LPS） stimulation; DHAP [DHAP injection, 3,4-DHAP 40 mg/piece （2 mL）, used for experiment, Beijing Third Pharmaceutical Factory] after 3 hours of pretreatment, plus LPS stimulation; simultaneous addition of DHAP and LPS; LPS after 3 hours of stimulation, plus DHAP. Other indexes were detected in three groups： control group （The cells were cultured normally and the medium of same quantity was added without any drug.）; DHAP pretreatment group （The cells were cultured for 3 hours in the appended medium dissolving 1×10^-7 mol/L DHAP, then stimulated in 10μg/L LPS for 4 hours and 8 hours.）; Unpretreated group （The cells were cultured for 3 hours in the appended medium of same quantity, then stimulated in 10 μg/L LPS for 4 hours and 8 hours）. ③Changes of sTLR4 and HO-lmRNA were analyzed with reverse transcription polymerase chain reaction （RT-PCR）; Change of sTLR4 protein level was detected with Western blot; Change of TNF-α secretion was detected with enzyme-linked immunoadsordent assay （ELISA）. ④The pair-matching t test was used to perform the data handling of groups. RESULTS： ①The expression of sTLR4 mRNA： After 4 hours of LPS stimulation, the expression was significantly lower in the unpretreated group than in t