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担子菌黄硬皮马勃农药活性的初步研究
  • ISSN号:1674-148X
  • 期刊名称:《青岛农业大学学报:自然科学版》
  • 时间:0
  • 分类:S481.9[农业科学—农药学;农业科学—植物保护]
  • 作者机构:[1]青岛农业大学植物保护学院,山东青岛266109, [2]西北农林科技大学理学院, [3]西北农林科技大学无公害农药研究中心
  • 相关基金:国家自然科学基金(No.30370019)、中国博士后科学研究基金(No.2003034417)、国家“十五”攻关(No.2002BA516A04)资助项目及莱阳农学院高层次人才基金(630610)资助项目
中文摘要:

用甲醇和氯仿等提取黄硬皮马勃Scleroderma citrinum Per.的子实体,获得提取物,并测定其杀虫、抑菌及除草活性,结果表明:黄硬皮马勃子实体提取物对粘虫具较拒食、毒杀和触杀作用,其中以拒食、毒杀活性较好,触杀活性相对较弱.黄硬皮马勃总浸膏(甲醇:氯仿=1:1提取)及其石油醚萃取物在25mg/ml的供试浓度下,对3龄粘虫的48h拒食率分别为96.8%、81.3%.石油醚萃取物饲喂3龄粘虫,LC50为8.4013mg/ml;触杀测定结果表明,25mg/ml的剂量对3龄粘虫48 h内的致死作用均小于54.0%.浓度为1mg/ml时,总浸膏及各萃取段对小麦赤霉病菌菌丝生长的抑制率在60%~70%;正丁醇萃取段对辣椒疫霉病菌、油菜菌核病菌菌丝生长抑制率大于85%.室内除草活性测定结果表明,总浸膏及各萃取段在浓度为12.5mg/ml时对反枝苋种子萌发、幼根和幼茎生长无明显抑制作用.

英文摘要:

Substances were extracted by methanol and chloroform ( methanol : chloroform = 1 : 1 ) from the fungus of Scleroderma citrinum Per. The bioactivities of the extracts against pests ( Mythimna separata, Fusarium graminearum, Phytophthora capsici, Sclerotinia sclerotiorum and Amaranthus retroflexus ) were tested. The extract showed high antifeedant and poisonous effect, but poor touch toxicity to 3^rd instars larvae of Mythimna separata. The antifeedant ratios of the total extract and the extract by petroleum to the 3^rd instars larvae 48 hr after feeding were 96.8% and 81.3% respectively. The LC50 of extract by petroleum to the 3^rd instars larvae was 8. 4013mg/ml 24hr after feeding. The mortality of the 3^rd instars larvae 48hr after touching extract by petroleum in concentration of 25. 0 mg/ml was 54%. The inhibit rates of the total extract against the growth of Fusarium graminearum ranged from 60% to 70%. At a concentration of 1 mg/ml, the inhibit rates of extract by butanol against the growth of Phytophthora capsici and Sclerotinia sclerotiorum were both higher than 85%. The total extract and extracts by three solvent did not show any bioactivity against seed germination of Amaranthus retroflexus at a concentration of 12.5 mg/ml.

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期刊信息
  • 《青岛农业大学学报:自然科学版》
  • 中国科技核心期刊
  • 主管单位:山东省教育厅
  • 主办单位:青岛农业大学
  • 主编:顾卫东
  • 地址:青岛市城阳区长城路700号
  • 邮编:266109
  • 邮箱:xbbjb@qau.edu.cn
  • 电话:0532-86080813 86080803
  • 国际标准刊号:ISSN:1674-148X
  • 国内统一刊号:ISSN:37-1459/N
  • 邮发代号:
  • 获奖情况:
  • 首届全国期刊展览印制质量二等奖(1990年),山东省优秀科技期刊,全国农业高校评比一等奖
  • 国内外数据库收录:
  • 中国中国科技核心期刊
  • 被引量:1692