中文摘要：

目的：研究白附子提取物对人SHG-44脑胶质瘤细胞生长的影响,初步探讨白附子对胶质瘤细胞增殖和凋亡的作用机制。方法：培养脑胶质瘤SHG-44细胞,将其分为空白对照组和8、40、200及1 000μg.L-1白附子提取物组,MTT法检测白附子提取物对SHG-44细胞生长的影响,倒置显微镜观察细胞凋亡形态,流式细胞术分析细胞周期和细胞凋亡率,细胞免疫组织化学法检测Bcl-2与Bax蛋白表达。结果：MTT结果,与空白对照组比较,8和200μg.L-1白附子提取物组细胞在30min和3h时,细胞增殖均明显受抑制,细胞增殖活性降低（P〈0.05）;1 000μg.L-1白附子提取物组细胞在30min、1h和3h时,细胞增殖活性均明显降低（P〈0.05）;不同浓度白附子提取物对胶质瘤细胞增殖抑制作用呈剂量-时间依赖性。镜下观察,与空白对照组比较,各药物组细胞均出现数量不等的凋亡小体。流式细胞术分析,部分细胞阻滞于G2/M期,细胞凋亡率随着药物浓度的增加而升高。细胞免疫组织化学检测,与空白对照组比较,200μg.L-1白附子提取物组细胞Bcl-2蛋白表达降低（P〈0.01）,Bax蛋白表达增高（P〈0.01）。结论：白附子提取物可抑制SHG-44细胞的增殖及诱导其发生凋亡,其作用机制与Bcl-2蛋白表达下降和Bax蛋白表达上升有关。

英文摘要：

Objective To study the influence of Rhizoma Typhonii extracts in glioma SHG-44cells and to explore the effects of Rhizoma Typhonii extracts on proliferation and apoptosis of glioma cells.Methods Glioma SHG-44 cells were cultured and divided into control and 8,40,200,and 1 000μg.L-1 Rhizoma Typhonii extracts groups,and the influence of Rhizoma Typhonii extracts in proliferation of SHG-44cells was measured by MTT assay.The morphology of apoptosis was observed with inverted microscope.The cell cycle and apoptotic rate were analyzed by flow cytometry（FCM）.The expressions of Bcl-2and Bax protein were detected by immunohistochemistry.Results Compared with control group,the poliferation of SHG-44cells was inhibited after treated with Rhizoma Typhonii extracts at concentrations of 8and 200μg.L-1 for 30min and 3hand the proliferation activities were decreased（P0.05）;in 1 000μg.L-1group after treated for 30min,1hand 3h,the proliferation activities were significantly decreased（P0.05）.The proliferation inhibition of different concentrations of Rhizoma Typhonii extracts was time-and dose-dependent.The apoptotic bodies were found under inverted microscope in different Rhizoma Typhonii extracts groups.The FCM results showed that part of cells were arrested at G2/M phase,and the apoptotic rates were increased with the increasing of drug concentrations.Cell immunohistochemisty showed that compared with control group the expression of Bcl-2protein in 200μg.L-1 Rhizoma Typhonii extracts groups was decreased（P0.01）and the expression of Bax protein was increased（P0.01）.Conclusion Rhizoma Typhonii extracts can inhibit the proliferation and induce apoptosis by down-regulating the Bcl-2protein expression and up-regulating the Bax protein expression in SHG-44cells.