中文摘要：

目的：探究P57KIP2基因过表达之后对滋养层细胞生物学行为的影响。方法：采用实时荧光定量（q PCR）及Western Blot方法检测人绒毛膜滋养层细胞（HTR8-/SVneo）过表达P57KIP2基因后mRNA和蛋白水平的表达变化;用Transwell方法检测HTR8-/SVneo细胞过表达P57KIP2基因后迁移、侵袭能力生物学行为的变化;CCK8检测HTR8-/SVneo细胞过表达该基因后增殖能力的变化。采用免疫荧光检测HTR8-/SVneo细胞过表达该基因后,抗凋亡蛋白Bcl2的表达变化。结果：滋养层细胞过表达P57KIP2基因后,其mRNA及蛋白水平均明显增高（P〈0.05）;P57KIP2基因过表达后滋养层细胞的迁移、侵袭数量均明显增高,差异有统计学意义（P〈0.05）;滋养层细胞的细胞相对吸光值48小时后明显增高（P〈0.001）;过表达P57KIP2基因后滋养层细胞中抗凋亡蛋白Bcl2表达增高（P〈0.05）。结论：P57KIP2基因可能会增加滋养层细胞迁移、侵袭以及增殖能力,P57KIP2基因可能参与调控滋养细胞的凋亡过程。

英文摘要：

Objective：To check the effect P57KIP2 overexpression on biological behaviors of trophoblast cell.Methods：After P57KIP2 overexpression,the mRNA and protein expression of P57KIP2 were detected by realtime fluorescence quantitative（q PCR） and Western Blot to detectin human trophoblast cell（HTR8-/SVneo）.The change of migration and invasion were test by Transwell method,the changes the proliferation was detected by CCK8;the change of the expression of anti-apoptotic protein Bcl2 was detected by immunofluorescence staining.Results：Overexpresion P57KIP2 significantly increased the relative mRNA and protein expression of P57KIP2gene（P0.05）;and the number of imgration and invasion of trophoblast cells were significantly increased（P 0.05）;and the proliferation of trophoblast cells was significantly enhanced after 48 hours（P0.001）;the expression of antiapoptotic protein Bcl2 was increased in human trophoblast cells（P0.05）.Conclusions：P57KIP2 gene may increase the ability of migration,invasion and proliferation of trophoblast cells.P57KIP2 gene may be involved in the regulation of apoptosis of trophoblast cell.