中文摘要：

本研究通过富勒烯与赖氨酸直接反应，合成了一种富勒烯赖氨酸衍生物（C60-Lys），并通过元素分析、红外光谱、1H．NMR、13C-NMR等对其分子结构进行了表征。细胞毒性研究发现C60-Lys对人淋巴母细胞AHH-1的毒性较小，在1200mg／L浓度以下的细胞存活率和未用药对照组相比无明显差异。辐射生物效应研究发现400mg／L以上C60—Lys预处理能显著提高受照细胞的存活率，并降低辐射诱发的凋亡率，其辐射保护效应具有明显的剂量依赖性。说明C60一Lys预处理对受4Gyγ射线照射的AHH-1细胞具有一定的保护作用。

英文摘要：

In this study, we synthesized a fullerene derivative C60-Lys via direct reaction of fullerene（C60） and Lysine, whose molecular structure was identified by elemental analysis, infrared spectrum, IH-NMR and 13C-NMR. It has been found that the chemical C60-Lys has little toxicity on human lymphoblast cell AHH-1. CCK-8 assay and AnnexinV/PI staining assay were performed to evaluate the cell survival rate and apoptosis after γ rays irradiation. The cell survival rate of C60-Lys treatment groups （≤1200 mg/L ） shows no significant difference compared with the control group. The results show that pretreatment of cell with C60-Lys（≥400 mg/L） markedly increases the cell survival and decreases the cell apoptosis rate after irradiation exposure in a dose-dependant manner. These results can be suggested that C60-Lys has certain radioprotection effects to AHH-1 cell against radiation induced damages.